TF Info Page for NPAS3 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Inferred motif 2 Obligate heteromer In vivo/Misc source Likely obligate heteromer (PMID: 9374395; PMID: 27782878).

Description

Description: neuronal PAS domain protein 3 [Source:HGNC Symbol;Acc:HGNC:19311]
Entrez Summary NA
Ensembl ID: ENSG00000151322
External Link: CisBP
Interpro IPR000014; IPR011598; IPR013655; IPR013767;
Protein Domain: ENSP00000319610
Protein Domain: ENSP00000348460
Protein Domain: ENSP00000350446
Protein Domain: ENSP00000449542
Protein Domain: ENSP00000448916
Protein Domain: ENSP00000447213
Protein Domain: ENSP00000446700
Domain:
Protein: ENSP00000319610DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000348460DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000350446DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000449542DBD: bHLHOther: PAS, PAS_3
Protein: ENSP00000448916DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000447213DBD: bHLHOther: PAS, PAS_3
Protein: ENSP00000446700DBD: bHLHOther: PAS

Previous Annotations

Source Annotation
TF-CAT classification TF Gene Candidate_DNA-Binding
sequence-specific_Other_
PMIDS:16172381 9374395 16172381 10534623 16172381 16190882
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
c
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a2, Orthologous HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredInferred - HIF1A (69% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (69% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (69% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (69% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (69% AA Identity, Homo sapiens)
MiscHocoMocoInferred - HIF1A (69% AA Identity, Homo sapiens)
MiscHOMERInferred - HIF1A (69% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - EPAS1 (64% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - EPAS1 (64% AA Identity, Homo sapiens)
MiscHocoMocoInferred - EPAS1 (64% AA Identity, Homo sapiens)
MiscHOMERInferred - EPAS1 (64% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Hif3a (61% AA Identity, Mus musculus)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution