TF Info Page for ZEB1 (C2H2 ZF; Homeodomain)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source

Description

Description: zinc finger E-box binding homeobox 1 [Source:HGNC Symbol;Acc:HGNC:11642]
Entrez Summary This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]
Ensembl ID: ENSG00000148516
External Link: CisBP
Interpro IPR001356; IPR007087; IPR008598; IPR009057; IPR015880; ;
Protein Domain: ENSP00000319248
Protein Domain: ENSP00000354487
Protein Domain: ENSP00000453970
Protein Domain: ENSP00000444891
Protein Domain: ENSP00000453674
Protein Domain: ENSP00000452787
Domain:
Protein: ENSP00000319248DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000354487DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000453970DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000444891DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000453674DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000452787DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:16129691
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001227
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IDA - PMID:23814079
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:8138542
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
ChIP-seqENCODEDirect
ChIP-seqJASPARDirect
ChIP-seqmodENCODEDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
SMiLE-seqIsakova2017Inferred - ZEB1 (98% AA Identity, Mus musculus)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution