TF Info Page for ARNT (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer 100 perc ID - in vitro

Description

Description: aryl hydrocarbon receptor nuclear translocator [Source:HGNC Symbol;Acc:HGNC:700]
Entrez Summary This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]
Ensembl ID: ENSG00000143437
External Link: CisBP
Interpro IPR000014; IPR001067; IPR001610; IPR011598; IPR013767; ;
Protein Domain: ENSP00000346372
Protein Domain: ENSP00000351407
Protein Domain: ENSP00000423851
Protein Domain: ENSP00000427571
Domain:
Protein: ENSP00000346372DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000351407DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000423851DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000427571DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:7592839
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:10777486, PMID:1317062
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
IDA - PMID:7539918, PMID:8756616
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements Obligate_Multimer, the structure4ZPR is a HIF1A-AR

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERDirect
PBMZoo_01Inferred - Arnt (100% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - ARNT2 (94% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Arnt2 (94% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - Arnt2 (94% AA Identity, Rattus norvegicus)
MiscHocoMocoInferred - ARNT2 (94% AA Identity, Homo sapiens)
SELEXJASPARInferred - Arnt (76% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - ARNTL (65% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ARNTL (65% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Arntl (65% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ARNTL (65% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Arntl (65% AA Identity, Rattus norvegicus)
MiscHocoMocoInferred - ARNTL (65% AA Identity, Homo sapiens)
MiscHOMERInferred - Arntl (65% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - cyc (60% AA Identity, Drosophila melanogaster)

Structure

Structure PDB 4ZPR

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution