TF Info Page for IRF8 (IRF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: interferon regulatory factor 8 [Source:HGNC Symbol;Acc:HGNC:5358]
Entrez Summary Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000140968
External Link: CisBP
Interpro IPR001346; IPR008984; IPR019471; IPR019817;
Protein Domain: ENSP00000268638
Protein Domain: ENSP00000458047
Protein Domain: ENSP00000456992
Protein Domain: ENSP00000455452
Protein Domain: ENSP00000455816
Protein Domain: ENSP00000455048
Protein Domain: ENSP00000455760
Protein Domain: ENSP00000456395
Domain:
Protein: ENSP00000268638DBD: Interferon regulatory factor (IRF)Other: IRF-3, MH2
Protein: ENSP00000458047DBD: Interferon regulatory factor (IRF)Other:
Protein: ENSP00000456992DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000455452DBD: Interferon regulatory factor (IRF)Other: IRF-3, MH2
Protein: ENSP00000455816DBD: Interferon regulatory factor (IRF)Other:
Protein: ENSP00000455048DBD: Interferon regulatory factor (IRF)Other:
Protein: ENSP00000455760DBD: Interferon regulatory factor (IRF)Other:
Protein: ENSP00000456395DBD: Interferon regulatory factor (IRF)Other: IRF-3, MH2

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:1460054 10734131
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
TAS - PMID:1460054
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
HT-SELEXYin2017Inferred - IRF4 (80% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - IRF4 (80% AA Identity, Homo sapiens)
PBMBadis09Inferred - Irf4 (80% AA Identity, Mus musculus)
ChIP-seqENCODEInferred - IRF4 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IRF4 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IRF4 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IRF4 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - IRF4 (80% AA Identity, Homo sapiens)
MiscHocoMocoInferred - IRF4 (80% AA Identity, Homo sapiens)
MiscHOMERInferred - IRF4 (80% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution