TF Info Page for NCOA2 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Likely to be sequence specific TF 2 Obligate heteromer No motif NCOA1/2/3 all have been tested on PBMs and HT-SELEX without yielding a motif. All are co-activators, collectively suggesting they might be obligate heteromers.

Description

Description: nuclear receptor coactivator 2 [Source:HGNC Symbol;Acc:HGNC:7669]
Entrez Summary The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Ensembl ID: ENSG00000140396
External Link: CisBP
Interpro IPR000014; IPR009110; IPR010011; IPR011598; IPR013767; IPR014920; IPR014935; IPR017426;
Protein Domain: ENSP00000399968
Protein Domain: ENSP00000429132
Protein Domain: ENSP00000430850
Protein Domain: ENSP00000430348
Domain:
Protein: ENSP00000399968DBD: bHLHOther: DUF1518, DUF4927, NCOA_u2, Nuc_rec_co-act, PAS, PA
Protein: ENSP00000429132DBD: bHLHOther: DUF1518, Nuc_rec_co-act
Protein: ENSP00000430850DBD: bHLHOther:
Protein: ENSP00000430348DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding_Co-activation_
PMIDS:14662770 15983373
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
x
CisBP considers it as a TF? No
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
3a, decent circumstantial evidence for its role as
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence

Structure

Structure PDB 3DZU

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution