The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

transcription factor 12 [Source:HGNC Symbol;Acc:HGNC:11623]

Entrez Summary

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Ensembl ID:

ENSG00000140262

External Link:

CisBP

Interpro

IPR011598; ;

Protein Domain:

ENSP00000267811

Protein Domain:

ENSP00000331057

Protein Domain:

ENSP00000342459

Protein Domain:

ENSP00000440017

Protein Domain:

ENSP00000453737

Protein Domain:

ENSP00000444696

Protein Domain:

ENSP00000453876

Protein Domain:

ENSP00000453264

Protein Domain:

ENSP00000456280

Protein Domain:

ENSP00000454823

Domain:

Protein: ENSP00000267811	DBD: bHLH	Other:
Protein: ENSP00000331057	DBD: bHLH	Other:
Protein: ENSP00000342459	DBD: bHLH	Other:
Protein: ENSP00000440017	DBD: bHLH	Other:
Protein: ENSP00000453737	DBD: bHLH	Other:
Protein: ENSP00000444696	DBD: bHLH	Other:
Protein: ENSP00000453876	DBD: bHLH	Other:
Protein: ENSP00000453264	DBD: bHLH	Other:
Protein: ENSP00000456280	DBD: bHLH	Other:
Protein: ENSP00000454823	DBD: bHLH	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:8163514 12826747
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IDA - PMID:11802795 GO:0003700 sequence-specific DNA binding transcription factor activity IDA - PMID:11802795
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
Misc	HOMER		Direct
PBM	Zoo_01		Inferred - Tcf12 (100% AA Identity, Mus musculus)
ChIP-seq	JASPAR		Inferred - Tcf12 (100% AA Identity, Mus musculus)
HT-SELEX	Jolma2010		Inferred - TCF4 (96% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - TCF4 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - TCF4 (96% AA Identity, Homo sapiens)
PBM	Zoo_01		Inferred - Tcf4 (96% AA Identity, Mus musculus)
SELEX	Hallikas2006		Inferred - Tcf4 (96% AA Identity, Mus musculus)
Misc	HOMER		Inferred - Tcf3 (96% AA Identity, Mus musculus)
Misc	HOMER		Inferred - Tcf3 (96% AA Identity, Mus musculus)
Misc	HocoMoco		Inferred - TCF4 (96% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - TCF4 (96% AA Identity, Homo sapiens)
SELEX	JASPAR		Inferred - TCF3 (92% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF3 (92% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF3 (92% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF3 (92% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF3 (92% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF3 (92% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - TCF3 (92% AA Identity, Homo sapiens)
PBM	Badis09		Inferred - Tcf3 (87% AA Identity, Mus musculus)
PBM	DREAM_contest		Inferred - Tcf3 (87% AA Identity, Mus musculus)
PBM	Zoo_01		Inferred - Tcf3 (87% AA Identity, Mus musculus)
HT-SELEX	Nitta2015		Inferred - da (75% AA Identity, Drosophila melanogaster)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for TCF12 (bHLH)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution