TF Info Page for ASCL1 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: achaete-scute family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:738]
Entrez Summary This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000139352
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000266744
Domain:
Protein: ENSP00000266744DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding
DNA-Binding
sequence-specific_DNA Binding
TF PPI
Transactivation_
PMIDS:8662987 8900141
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001078
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:11736660
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:10903890
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHOMERInferred - Ascl1 (100% AA Identity, Mus musculus)
PBMBadis09Inferred - Ascl2 (88% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - ASCL2 (86% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ASCL2 (86% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ASCL2 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ASCL2 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - ASCL2 (86% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - sc (54% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - ac (53% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution