The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	lymphoid enhancer binding factor 1 [Source:HGNC Symbol;Acc:HGNC:6551]
Entrez Summary	TBA
Ensembl ID:	ENSG00000138795
External Link:	T144096_1.02
Interpro	IPR009071; IPR013558;
Protein Domain:
Protein: ENSP00000265165	DBD: HMG	Other: CTNNB1_binding
Protein: ENSP00000369284	DBD: HMG	Other: CTNNB1_binding
Protein: ENSP00000406176	DBD: HMG	Other: CTNNB1_binding
Protein: ENSP00000422840	DBD: HMG	Other: CTNNB1_binding
Protein: ENSP00000427365	DBD: HMG	Other: CTNNB1_binding
Protein: ENSP00000422801	DBD: HMG	Other: CTNNB1_binding

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_TF PPI_ PMIDS:10958684 11696550 14579381
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IEA - GO_REF:0000019 GO:0001228 RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor IMP - PMID:23001182 GO:0003700 sequence-specific DNA binding transcription factor activity IDA - PMID:11751639 GO:0003705 RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity IBA - GO_REF:0000033
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
SMiLE-seq	Isakova2017		Direct
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
PBM	Badis09		Inferred - Lef1 (100% AA Identity, Mus musculus)
PBM	Badis09		Inferred - Tcf7l2 (98% AA Identity, Mus musculus)
ChIP-seq	JASPAR		Inferred - TCF7L2 (98% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF7L2 (98% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF7L2 (98% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF7L2 (98% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF7L2 (98% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF7L2 (98% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - TCF7L2 (98% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - TCF7L2 (98% AA Identity, Homo sapiens)
SMiLE-seq	Isakova2017		Inferred - TCF7 (97% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - TCF7 (97% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - TCF7 (97% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - TCF7 (97% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - TCF7 (97% AA Identity, Homo sapiens)
PBM	Badis09		Inferred - Tcf7 (97% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - TCF7 (97% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TCF7 (97% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - TCF7 (97% AA Identity, Homo sapiens)

Structure

Structure	PDB	2LEF

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for LEF1 (HMG/Sox)