TF Info Page for POU2F3 (Homeodomain; POU)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: POU class 2 homeobox 3 [Source:HGNC Symbol;Acc:HGNC:19864]
Entrez Summary This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Ensembl ID: ENSG00000137709
External Link: CisBP
Interpro IPR000327; IPR000972; IPR001356; IPR009057; IPR010982; IPR013847; IPR017970; ;
Protein Domain: ENSP00000441687
Protein Domain: ENSP00000260264
Protein Domain: ENSP00000453150
Domain:
Protein: ENSP00000441687DBD: Homeodomain,POUOther:
Protein: ENSP00000260264DBD: Homeodomain,POUOther:
Protein: ENSP00000453150DBD: Homeodomain,POUOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:12624109 | IMP - PMID:12624109
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
PBMBerger08Inferred - Pou2f3 (99% AA Identity, Mus musculus)
PBMZoo_01Inferred - pou2f3 (93% AA Identity, Takifugu rubripes)
PBMBerger06Inferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F1 (90% AA Identity, Homo sapiens)
MiscHocoMocoInferred - POU2F1 (90% AA Identity, Homo sapiens)
PBMBerger08Inferred - Pou2f1 (89% AA Identity, Mus musculus)
HT-SELEXJolma2010Inferred - POU2F2 (89% AA Identity, Homo sapiens)
HT-SELEXJolma2010Inferred - POU2F2 (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU2F2 (89% AA Identity, Homo sapiens)
PBMBerger08Inferred - Pou2f2 (89% AA Identity, Mus musculus)
ChIP-seqENCODEInferred - POU2F2 (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - POU2F2 (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - POU2F2 (89% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - POU2F2 (89% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - POU2F2 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F2 (89% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU2F2 (89% AA Identity, Homo sapiens)
MiscHocoMocoInferred - POU2F2 (89% AA Identity, Homo sapiens)
MiscHOMERInferred - Pou2f2 (89% AA Identity, Mus musculus)
PBMZoo_01Inferred - estExt_fgenesh2_pg.C_80253 (88% AA Identity, Branchiostoma floridae)
B1HJASPARInferred - nub (86% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - nub (86% AA Identity, Drosophila melanogaster)
MisciDMMPMMInferred - nub (86% AA Identity, Drosophila melanogaster)
PBMZoo_01Inferred - GB53100 (85% AA Identity, Apis mellifera)
PBMZoo_01Inferred - NV11360 (85% AA Identity, Nasonia vitripennis)
HT-SELEXNitta2015Inferred - pdm2 (84% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - pdm2 (84% AA Identity, Drosophila melanogaster)
PBMBarrera2016Inferred - POU3F4 (72% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F4 (72% AA Identity, Homo sapiens)
PBMBerger08Inferred - Pou3f4 (72% AA Identity, Mus musculus)
PBMZoo_01Inferred - WUBG_18372 (72% AA Identity, Wuchereria bancrofti)
PBMZoo_01Inferred - D2GWY1_AILME (71% AA Identity, Ailuropoda melanoleuca)
HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F2 (71% AA Identity, Homo sapiens)
PBMBerger08Inferred - Pou3f2 (71% AA Identity, Mus musculus)
PBMZoo_01Inferred - pou3f2a (71% AA Identity, Takifugu rubripes)
PBMZoo_01Inferred - pou3f3b (71% AA Identity, Danio rerio)
PBMBarrera2016Inferred - POU3F4 (71% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - POU3F4 (71% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - vvl (71% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - POU3F2 (71% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU3F2 (71% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU3F2 (71% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU3F2 (71% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU3F3 (71% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SGF3_BOMMO (71% AA Identity, Bombyx mori)
DNaseI footprintingJASPARInferred - vvl (71% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - vvl (71% AA Identity, Drosophila melanogaster)
MiscHocoMocoInferred - POU3F2 (71% AA Identity, Homo sapiens)
PBMZoo_01Inferred - AAH72060 (70% AA Identity, Xenopus laevis)
HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU3F1 (70% AA Identity, Homo sapiens)
PBMBerger08Inferred - Pou3f1 (70% AA Identity, Mus musculus)
PBMDREAM_contestInferred - Pou3f1 (70% AA Identity, Mus musculus)
PBMBerger08Inferred - Pou3f3 (70% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - POU3F1 (70% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU3F1 (70% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU3F1 (70% AA Identity, Homo sapiens)
MiscHocoMocoInferred - POU3F1 (70% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution