TF Info Page for TFAP2A (AP-2)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: transcription factor AP-2 alpha [Source:HGNC Symbol;Acc:HGNC:11742]
Entrez Summary The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]
Ensembl ID: ENSG00000137203
External Link: CisBP
Interpro IPR008121; IPR013854;
Protein Domain: ENSP00000368928
Protein Domain: ENSP00000368933
Protein Domain: ENSP00000417495
Protein Domain: ENSP00000417735
Protein Domain: ENSP00000418391
Protein Domain: ENSP00000419696
Domain:
Protein: ENSP00000368928DBD: AP-2Other:
Protein: ENSP00000368933DBD: AP-2Other:
Protein: ENSP00000417495DBD: AP-2Other:
Protein: ENSP00000417735DBD: AP-2Other:
Protein: ENSP00000418391DBD: AP-2Other:
Protein: ENSP00000419696DBD: AP-2Other:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:11522791 15475956
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IDA - PMID:7555706
GO:0000982
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:21084835
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:11278550, PMID:20808827
GO:0001078
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:11278550, PMID:20066163, PMID:9520389
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
ChIP-seqENCODEDirect
SELEXJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - Tfap2a (99% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - TFAP2B (89% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - TFAP2B (89% AA Identity, Homo sapiens)
PBMBadis09Inferred - Tfap2b (89% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - TFAP2B (89% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TFAP2B (89% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - TFAP2E (84% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - TFAP2E (84% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - TFAP2E (84% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - TFAP2E (84% AA Identity, Homo sapiens)
PBMBadis09Inferred - Tfap2e (84% AA Identity, Mus musculus)
PBMBadis09Inferred - Tfap2c (80% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - TFAP2C (79% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - TFAP2C (79% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - TFAP2C (79% AA Identity, Homo sapiens)
SELEXJASPARInferred - TFAP2C (79% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TFAP2C (79% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TFAP2C (79% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TFAP2C (79% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TFAP2C (79% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TFAP2C (79% AA Identity, Homo sapiens)
MiscHOMERInferred - TFAP2C (79% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TFAP2D (76% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution