TF Info Page for MYC (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 2 Obligate heteromer In vivo/Misc source Functions as a heterodimer with MAX.

Description

Description: v-myc avian myelocytomatosis viral oncogene homolog [Source:HGNC Symbol;Acc:HGNC:7553]
Entrez Summary This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
Ensembl ID: ENSG00000136997
External Link: CisBP
Interpro IPR002418; IPR003327; IPR011598; IPR012682;
Protein Domain: ENSP00000259523
Protein Domain: ENSP00000367207
Protein Domain: ENSP00000429441
Protein Domain: ENSP00000430235
Protein Domain: ENSP00000479618
Protein Domain: ENSP00000478887
Protein Domain: ENSP00000430226
Domain:
Protein: ENSP00000259523DBD: bHLHOther: Myc_N
Protein: ENSP00000367207DBD: bHLHOther: Myc_N, TRH
Protein: ENSP00000429441DBD: bHLHOther: Myc_N
Protein: ENSP00000430235DBD: bHLHOther: Myc_N, TRH
Protein: ENSP00000479618DBD: bHLHOther: Myc_N, TRH
Protein: ENSP00000478887DBD: bHLHOther: Myc_N, TRH
Protein: ENSP00000430226DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:12177005 16287840 16767079
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:10723141
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:9924025
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements Obligate_Multimer

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
ChIP-seqENCODEDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERDirect
ChIP-seqJASPARInferred - Myc (92% AA Identity, Mus musculus)
MiscHOMERInferred - Myc (92% AA Identity, Mus musculus)

Structure

Structure PDB 1NKP

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution