TF Info Page for KLF4 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Kruppel like factor 4 [Source:HGNC Symbol;Acc:HGNC:6348]
Entrez Summary This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Ensembl ID: ENSG00000136826
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000363804
Protein Domain: ENSP00000399921
Protein Domain: ENSP00000404922
Protein Domain: ENSP00000483629
Domain:
Protein: ENSP00000363804DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000399921DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000404922DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000483629DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info GO:0001010
sequence-specific DNA binding transcription factor recruiting transcription factor activity
ISS - GO_REF:0000024
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IMP - PMID:21539536
GO:0003700
sequence-specific DNA binding transcription factor activity
NAS - PMID:9422764
GO:0032088
negative regulation of NF-kappaB transcription factor activity
IDA - PMID:20551324
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
ChIP-seqNajafabadi2015Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
ChIP-seqhmCHIPInferred - Klf4 (100% AA Identity, Mus musculus)
ChIP-seqJASPARInferred - Klf4 (100% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - KLF2 (94% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF2 (94% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF2 (94% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (84% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - KLF1 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF1 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF1 (84% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF1 (84% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (83% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (83% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (83% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - Klf1 (83% AA Identity, Mus musculus)
MiscHOMERInferred - Klf1 (83% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - KLF5 (82% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF5 (82% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - KLF5 (82% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF5 (82% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF5 (82% AA Identity, Homo sapiens)
MiscHOMERInferred - KLF5 (82% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF3 (80% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF3 (80% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF3 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF3 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF3 (80% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF3 (80% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF12 (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF12 (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF12 (78% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Klf12 (78% AA Identity, Mus musculus)
PBMBadis09Inferred - Klf7 (78% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - luna (78% AA Identity, Drosophila melanogaster)
ChIP-seqSchmitges2016Inferred - KLF12 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF12 (78% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - KLF7 (78% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF6 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF6 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF6 (76% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF6 (76% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Klf8 (75% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - KLF8 (75% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF8 (75% AA Identity, Homo sapiens)
PBMCEPDInferred - klf-3 (72% AA Identity, Caenorhabditis elegans)

Structure

Structure PDB 2WBS

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution