The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	Kruppel like factor 4 [Source:HGNC Symbol;Acc:HGNC:6348]
Entrez Summary	TBA
Ensembl ID:	ENSG00000136826
External Link:	T044363_1.02
Interpro	IPR007087; IPR015880;
Protein Domain:
Protein: ENSP00000363804	DBD: C2H2 ZF Containing Proteins	Other:
Protein: ENSP00000399921	DBD: C2H2 ZF Containing Proteins	Other:
Protein: ENSP00000404922	DBD: C2H2 ZF Containing Proteins	Other:
Protein: ENSP00000483629	DBD: C2H2 ZF Containing Proteins	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	No PMIDS:
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	No
GO-Info	GO:0001010 sequence-specific DNA binding transcription factor recruiting transcription factor activity ISS - GO_REF:0000024 GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IMP - PMID:21539536 GO:0003700 sequence-specific DNA binding transcription factor activity NAS - PMID:9422764 GO:0032088 negative regulation of NF-kappaB transcription factor activity IDA - PMID:20551324
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
ChIP-seq	Najafabadi2015		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
ChIP-seq	hmCHIP		Inferred - Klf4 (100% AA Identity, Mus musculus)
ChIP-seq	JASPAR		Inferred - Klf4 (100% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - KLF2 (94% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - KLF2 (94% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF2 (94% AA Identity, Homo sapiens)
PBM	Barrera2016		Inferred - KLF1 (84% AA Identity, Homo sapiens)
ChIP-seq	Schmitges2016		Inferred - KLF1 (84% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF1 (84% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF1 (84% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - KLF1 (84% AA Identity, Homo sapiens)
PBM	Barrera2016		Inferred - KLF1 (83% AA Identity, Homo sapiens)
PBM	Barrera2016		Inferred - KLF1 (83% AA Identity, Homo sapiens)
PBM	Barrera2016		Inferred - KLF1 (83% AA Identity, Homo sapiens)
ChIP-seq	JASPAR		Inferred - Klf1 (83% AA Identity, Mus musculus)
Misc	HOMER		Inferred - Klf1 (83% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - KLF5 (82% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - KLF5 (82% AA Identity, Homo sapiens)
ChIP-seq	JASPAR		Inferred - KLF5 (82% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF5 (82% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF5 (82% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - KLF5 (82% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - KLF3 (80% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - KLF3 (80% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - KLF3 (80% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF3 (80% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF3 (80% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - KLF3 (80% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - KLF12 (78% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - KLF12 (78% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - KLF12 (78% AA Identity, Homo sapiens)
PBM	DREAM_contest		Inferred - Klf12 (78% AA Identity, Mus musculus)
PBM	Badis09		Inferred - Klf7 (78% AA Identity, Mus musculus)
HT-SELEX	Nitta2015		Inferred - luna (78% AA Identity, Drosophila melanogaster)
ChIP-seq	Schmitges2016		Inferred - KLF12 (78% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF12 (78% AA Identity, Homo sapiens)
ChIP-seq	Schmitges2016		Inferred - KLF7 (78% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - KLF6 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - KLF6 (76% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - KLF6 (76% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - KLF6 (76% AA Identity, Homo sapiens)
PBM	DREAM_contest		Inferred - Klf8 (75% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - KLF8 (75% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - KLF8 (75% AA Identity, Homo sapiens)
PBM	CEPD		Inferred - klf-3 (72% AA Identity, Caenorhabditis elegans)

Structure

Structure	PDB	2WBS

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for KLF4 (C2H2 ZF(non-KRAB))