TF Info Page for SKIL (Unknown)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Likely to be sequence specific TF 1 Monomer or homomultimer No motif Homodimerizes on GTCTAGAC sequence (EMSA) (PMID: 9824161)

Description

Description: SKI-like proto-oncogene [Source:HGNC Symbol;Acc:HGNC:10897]
Entrez Summary The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Ensembl ID: ENSG00000136603
External Link: CisBP
Interpro IPR003380; IPR009061; IPR010919; IPR014890;
Protein Domain: ENSP00000259119
Protein Domain: ENSP00000400193
Protein Domain: ENSP00000415243
Protein Domain: ENSP00000406520
Domain:
Protein: ENSP00000259119DBD: OtherOther: Ski_Sno, c-SKI_SMAD_bind
Protein: ENSP00000400193DBD: OtherOther: Ski_Sno, c-SKI_SMAD_bind
Protein: ENSP00000415243DBD: OtherOther: Ski_Sno, c-SKI_SMAD_bind
Protein: ENSP00000406520DBD: OtherOther: Ski_Sno, c-SKI_SMAD_bind

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding_DNA Binding
Transactivation_
PMIDS:9824161 9927733 10531062
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? No
TFclass considers it as a TF? No
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001078
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:10531062
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
4a, two or more datasets predict it as a TF
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution