The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

SRY-box 5 [Source:HGNC Symbol;Acc:HGNC:11201]

Entrez Summary

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Ensembl ID:

ENSG00000134532

External Link:

CisBP

Interpro

IPR009071; ;

Protein Domain:

ENSP00000379328

Protein Domain:

ENSP00000398273

Protein Domain:

ENSP00000437487

Protein Domain:

ENSP00000393240

Protein Domain:

ENSP00000441973

Protein Domain:

ENSP00000443520

Protein Domain:

ENSP00000442119

Domain:

Protein: ENSP00000379328	DBD: HMG	Other: CENP-K
Protein: ENSP00000398273	DBD: HMG	Other:
Protein: ENSP00000437487	DBD: HMG	Other:
Protein: ENSP00000393240	DBD: HMG	Other:
Protein: ENSP00000441973	DBD: HMG	Other:
Protein: ENSP00000443520	DBD: HMG	Other:
Protein: ENSP00000442119	DBD: HMG	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:1396566 8078769 9755172
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0003700 sequence-specific DNA binding transcription factor activity TAS - PMID:8812465
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
SMiLE-seq	Isakova2017		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
PBM	Badis09		Inferred - Sox5 (100% AA Identity, Mus musculus)
SELEX	JASPAR		Inferred - Sox5 (100% AA Identity, Mus musculus)
PBM	Badis09		Inferred - Sox13 (94% AA Identity, Mus musculus)
Misc	HocoMoco		Inferred - SOX13 (94% AA Identity, Homo sapiens)
SMiLE-seq	Isakova2017		Inferred - SOX6 (92% AA Identity, Homo sapiens)
PBM	DREAM_contest		Inferred - Sox6 (92% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - SOX6 (92% AA Identity, Homo sapiens)
ChIP-seq	JASPAR		Inferred - Sox6 (92% AA Identity, Mus musculus)
Misc	HOMER		Inferred - Sox6 (92% AA Identity, Mus musculus)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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{"regions": [{"startStyle": "curved", "end": 624, "endStyle": "curved", "aliStart": 556, "text": "HMG", "colour": "#228B22", "aliEnd": 624, "start": 556, "href": "http://pfam.xfam.org/family/PF00505.17", "type": "pfama", "display": "true", "metadata": {"end": 624, "description": "High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. The structure of the HMG-box domain consists of three helices in an irregular array. HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. Many of these proteins are regulators of gene expression. HMG-box proteins are found in a variety of eukaryotic organisms, and can be broadly divided into two groups, based on sequence-dependent and sequence-independent DNA recognition; the former usually contain one HMG-box motif, while the latter can contain multiple HMG-box motifs.", "database": "PfamA", "aliStart": 556, "scoreName": "E-value", "accession": "PF00505.17", "start": 556, "score": 2.4e-22, "identifier": "HMG (high mobility group) box", "type": "DBD", "aliEnd": 624}}], "length": 764}

{"regions": [{"startStyle": "curved", "end": 611, "endStyle": "curved", "aliStart": 543, "text": "HMG", "colour": "#228B22", "aliEnd": 611, "start": 543, "href": "http://pfam.xfam.org/family/PF00505.17", "type": "pfama", "display": "true", "metadata": {"end": 611, "description": "High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. The structure of the HMG-box domain consists of three helices in an irregular array. HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. Many of these proteins are regulators of gene expression. HMG-box proteins are found in a variety of eukaryotic organisms, and can be broadly divided into two groups, based on sequence-dependent and sequence-independent DNA recognition; the former usually contain one HMG-box motif, while the latter can contain multiple HMG-box motifs.", "database": "PfamA", "aliStart": 543, "scoreName": "E-value", "accession": "PF00505.17", "start": 543, "score": 2.3e-22, "identifier": "HMG (high mobility group) box", "type": "DBD", "aliEnd": 611}}], "length": 751}

{"regions": [{"startStyle": "curved", "end": 503, "endStyle": "curved", "aliStart": 435, "text": "HMG", "colour": "#228B22", "aliEnd": 503, "start": 435, "href": "http://pfam.xfam.org/family/PF00505.17", "type": "pfama", "display": "true", "metadata": {"end": 503, "description": "High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. The structure of the HMG-box domain consists of three helices in an irregular array. HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. Many of these proteins are regulators of gene expression. HMG-box proteins are found in a variety of eukaryotic organisms, and can be broadly divided into two groups, based on sequence-dependent and sequence-independent DNA recognition; the former usually contain one HMG-box motif, while the latter can contain multiple HMG-box motifs.", "database": "PfamA", "aliStart": 435, "scoreName": "E-value", "accession": "PF00505.17", "start": 435, "score": 1.9e-22, "identifier": "HMG (high mobility group) box", "type": "DBD", "aliEnd": 503}}], "length": 643}

{"regions": [{"startStyle": "curved", "end": 614, "endStyle": "curved", "aliStart": 546, "text": "HMG", "colour": "#228B22", "aliEnd": 614, "start": 546, "href": "http://pfam.xfam.org/family/PF00505.17", "type": "pfama", "display": "true", "metadata": {"end": 614, "description": "High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. The structure of the HMG-box domain consists of three helices in an irregular array. HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. Many of these proteins are regulators of gene expression. HMG-box proteins are found in a variety of eukaryotic organisms, and can be broadly divided into two groups, based on sequence-dependent and sequence-independent DNA recognition; the former usually contain one HMG-box motif, while the latter can contain multiple HMG-box motifs.", "database": "PfamA", "aliStart": 546, "scoreName": "E-value", "accession": "PF00505.17", "start": 546, "score": 2.3e-22, "identifier": "HMG (high mobility group) box", "type": "DBD", "aliEnd": 614}}], "length": 754}

TF Info Page for SOX5 (HMG/Sox)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution