TF Info Page for MEIS2 (Homeodomain)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Meis homeobox 2 [Source:HGNC Symbol;Acc:HGNC:7001]
Entrez Summary This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000134138
External Link: CisBP
Interpro IPR001356; IPR008422; IPR009057; ;
Protein Domain: ENSP00000341400
Protein Domain: ENSP00000339549
Protein Domain: ENSP00000404185
Protein Domain: ENSP00000453793
Protein Domain: ENSP00000380745
Protein Domain: ENSP00000453390
Protein Domain: ENSP00000452874
Protein Domain: ENSP00000475660
Protein Domain: ENSP00000475899
Domain:
Protein: ENSP00000341400DBD: HomeodomainOther: Meis_PKNOX_N
Protein: ENSP00000339549DBD: HomeodomainOther: Meis_PKNOX_N
Protein: ENSP00000404185DBD: HomeodomainOther: Meis_PKNOX_N
Protein: ENSP00000453793DBD: HomeodomainOther: Meis_PKNOX_N
Protein: ENSP00000380745DBD: HomeodomainOther: Meis_PKNOX_N
Protein: ENSP00000453390DBD: HomeodomainOther: Meis_PKNOX_N
Protein: ENSP00000452874DBD: HomeodomainOther: Meis_PKNOX_N
Protein: ENSP00000475660DBD: HomeodomainOther: Meis_PKNOX_N
Protein: ENSP00000475899DBD: HomeodomainOther: Meis_PKNOX_N

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:9811838 10764806
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:10764806
GO:0003700
sequence-specific DNA binding transcription factor activity
NAS - PMID:10764806
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2010Direct
HT-SELEXJolma2010Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
MiscHocoMocoDirect
PBMBerger08Inferred - Meis2 (100% AA Identity, Mus musculus)
PBMBerger08Inferred - Meis1 (98% AA Identity, Mus musculus)
SELEXJASPARInferred - MEIS1 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEIS1 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEIS1 (98% AA Identity, Homo sapiens)
MiscHocoMocoInferred - MEIS1 (98% AA Identity, Homo sapiens)
MiscHOMERInferred - Meis1 (98% AA Identity, Mus musculus)
B1HJASPARInferred - hth (96% AA Identity, Drosophila melanogaster)
PBMBerger08Inferred - Meis3 (96% AA Identity, Mus musculus)
PBMZoo_01Inferred - unc-62 (82% AA Identity, Caenorhabditis elegans)
PBMZoo_01Inferred - Cbr-unc-62 (74% AA Identity, Caenorhabditis briggsae)
HT-SELEXYin2017Inferred - PKNOX2 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - PKNOX2 (72% AA Identity, Homo sapiens)
PBMBerger08Inferred - Pknox2 (72% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - PKNOX1 (70% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - PKNOX1 (70% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - PKNOX1 (70% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - PKNOX1 (70% AA Identity, Homo sapiens)
PBMBerger08Inferred - Pknox1 (70% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - PKNOX1 (70% AA Identity, Homo sapiens)
MiscHocoMocoInferred - PKNOX1 (70% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Q5EVH4_OIKDI (65% AA Identity, Oikopleura dioica)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution