TF Info Page for BHLHE40 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: basic helix-loop-helix family member e40 [Source:HGNC Symbol;Acc:HGNC:1046]
Entrez Summary NA
Ensembl ID: ENSG00000134107
External Link: CisBP
Interpro IPR003650; IPR011598;
Protein Domain: ENSP00000256495
Domain:
Protein: ENSP00000256495DBD: bHLHOther: Hairy_orange

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding
DNA-Binding
sequence-specific_DNA Binding_
PMIDS:12297495 12397359
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IDA - PMID:12397359
GO:0001078
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:12624110, PMID:15193144, PMID:18411297
GO:0003700
sequence-specific DNA binding transcription factor activity
NAS - PMID:9240428
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
ISS - GO_REF:0000024
GO:0043433
negative regulation of sequence-specific DNA binding transcription factor activity
IDA - PMID:18411297
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
ChIP-seqENCODEDirect
SELEXJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERDirect
TransfacTransfacLicense requiredInferred - Bhlhe40 (100% AA Identity, Mus musculus)
PBMBadis09Inferred - Bhlhe40 (98% AA Identity, Mus musculus)
PBMZoo_01Inferred - Bhlhe40 (98% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - BHLHE41 (98% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHE41 (98% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Bhlhe41 (98% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - BHLHE41 (98% AA Identity, Homo sapiens)
MiscHocoMocoInferred - BHLHE41 (98% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution