TF Info Page for ARNTL (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: aryl hydrocarbon receptor nuclear translocator like [Source:HGNC Symbol;Acc:HGNC:701]
Entrez Summary The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Ensembl ID: ENSG00000133794
External Link: CisBP
Interpro IPR000014; IPR001067; IPR001610; IPR011598; IPR013767; ;
Protein Domain: ENSP00000374357
Protein Domain: ENSP00000385915
Protein Domain: ENSP00000384517
Protein Domain: ENSP00000385897
Protein Domain: ENSP00000385581
Protein Domain: ENSP00000436721
Domain:
Protein: ENSP00000374357DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000385915DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000384517DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000385897DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000385581DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9
Protein: ENSP00000436721DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
TF PPI_
PMIDS:9576906 9616112
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000982
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
ISS - GO_REF:0000024
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMZoo_01Inferred - Arntl (100% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - Arntl (100% AA Identity, Rattus norvegicus)
MiscHOMERInferred - Arntl (100% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - cyc (66% AA Identity, Drosophila melanogaster)
PBMZoo_01Inferred - Arnt (65% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ARNT (65% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ARNT (65% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ARNT (65% AA Identity, Homo sapiens)
MiscHocoMocoInferred - ARNT (65% AA Identity, Homo sapiens)
MiscHOMERInferred - ARNT (65% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - ARNT2 (61% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Arnt2 (61% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - Arnt2 (61% AA Identity, Rattus norvegicus)
MiscHocoMocoInferred - ARNT2 (61% AA Identity, Homo sapiens)
SELEXJASPARInferred - Arnt (51% AA Identity, Mus musculus)

Structure

Structure PDB 4H10

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution