TF Info Page for NFATC1 (Rel)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: nuclear factor of activated T-cells 1 [Source:HGNC Symbol;Acc:HGNC:7775]
Entrez Summary The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]
Ensembl ID: ENSG00000131196
External Link: CisBP
Interpro IPR002909; IPR008366; IPR008967; IPR011539; IPR014756; ;
Protein Domain: ENSP00000253506
Protein Domain: ENSP00000316553
Protein Domain: ENSP00000327850
Protein Domain: ENSP00000380892
Protein Domain: ENSP00000389377
Protein Domain: ENSP00000442435
Protein Domain: ENSP00000466489
Protein Domain: ENSP00000468111
Protein Domain: ENSP00000466194
Protein Domain: ENSP00000467181
Protein Domain: ENSP00000439992
Domain:
Protein: ENSP00000253506DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000316553DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000327850DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000380892DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000389377DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000442435DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000466489DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000468111DBD: RHDOther:
Protein: ENSP00000466194DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000467181DBD: RHDOther: RHD_dimer, TIG
Protein: ENSP00000439992DBD: RHDOther: RHD_dimer, TIG

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
TF PPI
Transactivation_
PMIDS:15117818 16109714
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001205
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involv
ISS - GO_REF:0000024
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:10821850, PMID:9506523
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
ISS - GO_REF:0000024
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2010Direct
HT-SELEXJolma2010Direct
HT-SELEXJolma2010Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHocoMocoDirect
MiscHOMERDirect
PBMZoo_01Inferred - NFATC1 (95% AA Identity, Meleagris gallopavo)
PBMZoo_01Inferred - NFATC1 (95% AA Identity, Monodelphis domestica)
PBMZoo_01Inferred - nfatc1 (93% AA Identity, Xenopus tropicalis)
HT-SELEXYin2017Inferred - NFATC2 (83% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NFATC2 (83% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFATC2 (83% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFATC2 (83% AA Identity, Homo sapiens)
PBMZoo_01Inferred - NFATC2 (83% AA Identity, Homo sapiens)
COMPILEDJASPARInferred - NFATC2 (83% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFATC2 (83% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFATC2 (83% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFATC2 (83% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NFATC2 (83% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NFATC3 (81% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NFATC3 (81% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NFATC3 (81% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFATC3 (81% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFATC3 (81% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFATC3 (81% AA Identity, Homo sapiens)
PBMZoo_01Inferred - NFATC3 (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFATC3 (81% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NFATC3 (81% AA Identity, Homo sapiens)
PBMTF_EvolutionUnpublished DataInferred - NFATC4 (78% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NFATC4 (78% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NFATC4 (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFATC4 (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFATC4 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFATC4 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFATC4 (78% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NFATC4 (78% AA Identity, Homo sapiens)
PBMZoo_01Inferred - NFATC2 (75% AA Identity, Tetraodon nigroviridis)

Structure

Structure PDB 1A66

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution