TF Info Page for MBD4 (MBD)


Assessment Binding Mode Motif Status Notes Comments
Likely to be sequence specific TF 3 Low specificity DNA-binding protein No motif


Description: methyl-CpG binding domain 4, DNA glycosylase [Source:HGNC Symbol;Acc:HGNC:6919]
Entrez Summary The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Ensembl ID: ENSG00000129071
External Link: CisBP
Interpro IPR001739; IPR003265; IPR011257; IPR016177; IPR017352; ;
Protein Domain: ENSP00000249910
Protein Domain: ENSP00000376959
Protein Domain: ENSP00000394080
Protein Domain: ENSP00000424873
Protein Domain: ENSP00000422327
Protein: ENSP00000249910DBD: Methyl-CpG DNA-bindingOther: HhH-GPD
Protein: ENSP00000376959DBD: Methyl-CpG DNA-bindingOther: HhH-GPD
Protein: ENSP00000394080DBD: Methyl-CpG DNA-bindingOther: HhH-GPD
Protein: ENSP00000424873DBD: Methyl-CpG DNA-bindingOther: HhH-GPD
Protein: ENSP00000422327DBD: Methyl-CpG DNA-bindingOther: HhH-GPD

Previous Annotations

Source Annotation
TF-CAT classification No
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
CisBP considers it as a TF? Yes
TFclass considers it as a TF? No
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements


Published Motif Data

Source Annotation Motif Evidence


Structure PDB 2MOE

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution