TF Info Page for KLF2 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Kruppel like factor 2 [Source:HGNC Symbol;Acc:HGNC:6347]
Entrez Summary This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is expressed early in mammalian development and is found in many different cell types. The protein acts to bind the CACCC box found in the promoter of target genes to activate their transcription. It plays a role in many processes during development and disease including adipogenesis, embryonic erythropoiesis, epithelial integrity, inflammation and t-cell viability. [provided by RefSeq, Mar 2017]
Ensembl ID: ENSG00000127528
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000248071
Protein Domain: ENSP00000465035
Domain:
Protein: ENSP00000248071DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000465035DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:21063504
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
HT-SELEXYin2017Inferred - KLF4 (94% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF4 (94% AA Identity, Homo sapiens)
ChIP-seqNajafabadi2015Inferred - KLF4 (94% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF4 (94% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF4 (94% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF4 (94% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF4 (94% AA Identity, Homo sapiens)
ChIP-seqhmCHIPInferred - Klf4 (94% AA Identity, Mus musculus)
ChIP-seqJASPARInferred - Klf4 (94% AA Identity, Mus musculus)
MiscHocoMocoInferred - KLF4 (94% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (84% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - KLF1 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF1 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF1 (84% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - Klf1 (84% AA Identity, Mus musculus)
MiscHocoMocoInferred - KLF1 (84% AA Identity, Homo sapiens)
MiscHOMERInferred - Klf1 (84% AA Identity, Mus musculus)
PBMBarrera2016Inferred - KLF1 (83% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (83% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (83% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF5 (80% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF5 (80% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - KLF5 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF5 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF5 (80% AA Identity, Homo sapiens)
MiscHOMERInferred - KLF5 (80% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF3 (79% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF3 (79% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF3 (79% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF3 (79% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF3 (79% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF3 (79% AA Identity, Homo sapiens)
PBMBadis09Inferred - Klf7 (78% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - luna (78% AA Identity, Drosophila melanogaster)
ChIP-seqSchmitges2016Inferred - KLF7 (78% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF12 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF12 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF12 (76% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Klf12 (76% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - KLF6 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF6 (76% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - KLF12 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF12 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF6 (76% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF6 (76% AA Identity, Homo sapiens)
PBMCEPDInferred - klf-3 (73% AA Identity, Caenorhabditis elegans)
PBMDREAM_contestInferred - Klf8 (73% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - KLF8 (73% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF8 (73% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution