TF Info Page for IRF3 (IRF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: interferon regulatory factor 3 [Source:HGNC Symbol;Acc:HGNC:6118]
Entrez Summary This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Ensembl ID: ENSG00000126456
External Link: CisBP
Interpro IPR001346; IPR008984; IPR019471; IPR019817;
Protein Domain: ENSP00000310127
Protein Domain: ENSP00000366339
Protein Domain: ENSP00000366344
Protein Domain: ENSP00000400378
Protein Domain: ENSP00000472601
Protein Domain: ENSP00000470512
Protein Domain: ENSP00000470069
Protein Domain: ENSP00000471358
Protein Domain: ENSP00000472700
Protein Domain: ENSP00000471896
Protein Domain: ENSP00000471517
Protein Domain: ENSP00000469113
Protein Domain: ENSP00000471402
Protein Domain: ENSP00000470140
Protein Domain: ENSP00000473176
Protein Domain: ENSP00000469067
Protein Domain: ENSP00000468911
Protein Domain: ENSP00000472223
Protein Domain: ENSP00000470905
Domain:
Protein: ENSP00000310127DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000366339DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000366344DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000400378DBD: Interferon regulatory factor (IRF)Other:
Protein: ENSP00000472601DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000470512DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000470069DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000471358DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000472700DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000471896DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000471517DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000469113DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000471402DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000470140DBD: Interferon regulatory factor (IRF)Other:
Protein: ENSP00000473176DBD: Interferon regulatory factor (IRF)Other:
Protein: ENSP00000469067DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000468911DBD: Interferon regulatory factor (IRF)Other:
Protein: ENSP00000472223DBD: Interferon regulatory factor (IRF)Other: IRF-3
Protein: ENSP00000470905DBD: Interferon regulatory factor (IRF)Other:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:9660935
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
IEA - GO_REF:0000002
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - Irf3 (85% AA Identity, Mus musculus)

Structure

Structure PDB 2O6G

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution