TF Info Page for MAX (bHLH)


Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro


Description: MYC associated factor X [Source:HGNC Symbol;Acc:HGNC:6913]
Entrez Summary The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Ensembl ID: ENSG00000125952
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000246163
Protein Domain: ENSP00000284165
Protein Domain: ENSP00000351175
Protein Domain: ENSP00000351490
Protein Domain: ENSP00000452286
Protein Domain: ENSP00000480127
Protein Domain: ENSP00000342482
Protein Domain: ENSP00000450818
Protein Domain: ENSP00000452378
Protein Domain: ENSP00000450955
Protein: ENSP00000246163DBD: bHLHOther:
Protein: ENSP00000284165DBD: bHLHOther:
Protein: ENSP00000351175DBD: bHLHOther:
Protein: ENSP00000351490DBD: bHLHOther:
Protein: ENSP00000452286DBD: bHLHOther:
Protein: ENSP00000480127DBD: bHLHOther:
Protein: ENSP00000342482DBD: bHLHOther:
Protein: ENSP00000450818DBD: bHLHOther:
Protein: ENSP00000452378DBD: bHLHOther:
Protein: ENSP00000450955DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements


Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
HT-SELEXNitta2015Inferred - Max (76% AA Identity, Drosophila melanogaster)
PBMBadis09Inferred - Max (62% AA Identity, Mus musculus)
PBMBadis09Inferred - Max (62% AA Identity, Mus musculus)
PBMZoo_01Inferred - Max (62% AA Identity, Mus musculus)
PBMGrove09Inferred - mxl-3 (61% AA Identity, Caenorhabditis elegans)


Structure PDB 1AN2

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution