TF Info Page for SPDEF (Ets)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: SAM pointed domain containing ETS transcription factor [Source:HGNC Symbol;Acc:HGNC:17257]
Entrez Summary The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Ensembl ID: ENSG00000124664
External Link: CisBP
Interpro IPR000418; IPR003118; IPR013761; ;
Protein Domain: ENSP00000363149
Protein Domain: ENSP00000442715
Domain:
Protein: ENSP00000363149DBD: ETSOther: SAM_PNT
Protein: ENSP00000442715DBD: ETSOther: SAM_PNT

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific
Transcription Factor Binding
tf co-factor binding_DNA Binding
Transactivation_
PMIDS:10625666
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
MiscHOMERDirect
PBMBadis09Inferred - Spdef (98% AA Identity, Mus musculus)
PBMWei10Inferred - Spdef (98% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - Ets98B (74% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Ets98B (74% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Ets98B (74% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Ets98B (74% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Ets98B (74% AA Identity, Drosophila melanogaster)

Structure

Structure PDB 1YO5

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution