The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Inferred motif	1 Monomer or homomultimer	In vivo/Misc source

Description

Description:	recombination signal binding protein for immunoglobulin kappa J region like [Source:HGNC Symbol;Acc:
Entrez Summary	TBA
Ensembl ID:	ENSG00000124232
External Link:
Interpro	IPR008967; IPR014756; IPR015350; IPR015351;
Protein Domain:
Protein: ENSP00000361826	DBD: LAG1, DNA binding	Other: BTD
Protein: ENSP00000361828	DBD: LAG1, DNA binding	Other: BTD
Protein: ENSP00000341243	DBD: LAG1, DNA binding	Other: BTD
Protein: ENSP00000483978	DBD: LAG1, DNA binding	Other: BTD

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:9111338 9111338 9111338 9111338 16354684
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0000982 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IEA - GO_REF:0000002 GO:0003700 sequence-specific DNA binding transcription factor activity TAS - PMID:9929984
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	3a, decent circumstantial evidence for its role as
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
Misc	HOMER		Inferred - Rbpjl (92% AA Identity, Mus musculus)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 206, "endStyle": "curved", "aliStart": 69, "text": "LAG1", "colour": "#009900", "aliEnd": 206, "start": 69, "href": "http://pfam.xfam.org/family/PF09271.9", "type": "pfama", "display": "true", "metadata": {"end": 206, "description": "Members of this family are found in various eukaryotic hypothetical proteins and in the DNA-binding protein LAG-1. They adopt a beta sandwich structure, with nine strands in two beta-sheets, in a Greek-key topology, and allow for DNA binding [1].  This domain is also known as RHR-N (Rel-homology region) as it related to Rel domain proteins.", "database": "PfamA", "aliStart": 69, "scoreName": "E-value", "accession": "PF09271.9", "start": 69, "score": 4.6e-44, "identifier": "LAG1, DNA binding", "type": "DBD", "aliEnd": 206}}, {"startStyle": "straight", "end": 359, "endStyle": "straight", "aliStart": 207, "text": "BTD", "colour": "#9999ff", "aliEnd": 359, "start": 207, "href": "http://pfam.xfam.org/family/PF09270.8", "type": "pfama", "display": "true", "metadata": {"end": 359, "description": "Members of this family of DNA binding domains adopt a beta-trefoil fold, that is, a capped beta-barrel with internal pseudo threefold symmetry. In the DNA-binding protein LAG-1, it also is the site of mutually exclusive interactions with NotchIC (and the viral protein EBNA2) and co-repressors (SMRT/N-Cor and CIR) [1].", "database": "PfamA", "aliStart": 207, "scoreName": "E-value", "accession": "PF09270.8", "start": 207, "score": 2.5e-62, "identifier": "Beta-trefoil DNA-binding domain", "type": "DBD", "aliEnd": 359}}], "length": 472}

{"regions": [{"startStyle": "curved", "end": 206, "endStyle": "curved", "aliStart": 69, "text": "LAG1", "colour": "#009900", "aliEnd": 206, "start": 69, "href": "http://pfam.xfam.org/family/PF09271.9", "type": "pfama", "display": "true", "metadata": {"end": 206, "description": "Members of this family are found in various eukaryotic hypothetical proteins and in the DNA-binding protein LAG-1. They adopt a beta sandwich structure, with nine strands in two beta-sheets, in a Greek-key topology, and allow for DNA binding [1].  This domain is also known as RHR-N (Rel-homology region) as it related to Rel domain proteins.", "database": "PfamA", "aliStart": 69, "scoreName": "E-value", "accession": "PF09271.9", "start": 69, "score": 5.699999999999999e-44, "identifier": "LAG1, DNA binding", "type": "DBD", "aliEnd": 206}}, {"startStyle": "straight", "end": 359, "endStyle": "straight", "aliStart": 207, "text": "BTD", "colour": "#9999ff", "aliEnd": 359, "start": 207, "href": "http://pfam.xfam.org/family/PF09270.8", "type": "pfama", "display": "true", "metadata": {"end": 359, "description": "Members of this family of DNA binding domains adopt a beta-trefoil fold, that is, a capped beta-barrel with internal pseudo threefold symmetry. In the DNA-binding protein LAG-1, it also is the site of mutually exclusive interactions with NotchIC (and the viral protein EBNA2) and co-repressors (SMRT/N-Cor and CIR) [1].", "database": "PfamA", "aliStart": 207, "scoreName": "E-value", "accession": "PF09270.8", "start": 207, "score": 2.9999999999999997e-62, "identifier": "Beta-trefoil DNA-binding domain", "type": "DBD", "aliEnd": 359}}], "length": 517}

{"regions": [{"startStyle": "curved", "end": 206, "endStyle": "curved", "aliStart": 69, "text": "LAG1", "colour": "#009900", "aliEnd": 206, "start": 69, "href": "http://pfam.xfam.org/family/PF09271.9", "type": "pfama", "display": "true", "metadata": {"end": 206, "description": "Members of this family are found in various eukaryotic hypothetical proteins and in the DNA-binding protein LAG-1. They adopt a beta sandwich structure, with nine strands in two beta-sheets, in a Greek-key topology, and allow for DNA binding [1].  This domain is also known as RHR-N (Rel-homology region) as it related to Rel domain proteins.", "database": "PfamA", "aliStart": 69, "scoreName": "E-value", "accession": "PF09271.9", "start": 69, "score": 5.699999999999999e-44, "identifier": "LAG1, DNA binding", "type": "DBD", "aliEnd": 206}}, {"startStyle": "straight", "end": 359, "endStyle": "straight", "aliStart": 207, "text": "BTD", "colour": "#9999ff", "aliEnd": 359, "start": 207, "href": "http://pfam.xfam.org/family/PF09270.8", "type": "pfama", "display": "true", "metadata": {"end": 359, "description": "Members of this family of DNA binding domains adopt a beta-trefoil fold, that is, a capped beta-barrel with internal pseudo threefold symmetry. In the DNA-binding protein LAG-1, it also is the site of mutually exclusive interactions with NotchIC (and the viral protein EBNA2) and co-repressors (SMRT/N-Cor and CIR) [1].", "database": "PfamA", "aliStart": 207, "scoreName": "E-value", "accession": "PF09270.8", "start": 207, "score": 2.9999999999999997e-62, "identifier": "Beta-trefoil DNA-binding domain", "type": "DBD", "aliEnd": 359}}], "length": 518}

TF Info Page for RBPJL (CSL)