TF Info Page for NCOA3 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Likely to be sequence specific TF 2 Obligate heteromer No motif NCOA1/2/3 all have been tested on PBMs and HT-SELEX without yielding a motif. All are co-activators, collectively suggesting they might be obligate heteromers.

Description

Description: nuclear receptor coactivator 3 [Source:HGNC Symbol;Acc:HGNC:7670]
Entrez Summary The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010]
Ensembl ID: ENSG00000124151
External Link: CisBP
Interpro IPR000014; IPR009110; IPR010011; IPR011598; IPR013767; IPR014920; IPR014935; IPR017426;
Protein Domain: ENSP00000361065
Protein Domain: ENSP00000361066
Protein Domain: ENSP00000361073
Domain:
Protein: ENSP00000361065DBD: bHLHOther: DUF1518, DUF4927, NCOA_u2, Nuc_rec_co-act, PAS, PA
Protein: ENSP00000361066DBD: bHLHOther: DUF1518, DUF4927, NCOA_u2, Nuc_rec_co-act, PAS, PA
Protein: ENSP00000361073DBD: bHLHOther: DUF1518, DUF4927, NCOA_u2, Nuc_rec_co-act, PAS, PA

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding_Co-activation_
PMIDS:9192892
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
c
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
4a, two or more datasets predict it as a TF
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution