TF Info Page for NR4A1 (Nuclear receptor)


Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro


Description: nuclear receptor subfamily 4 group A member 1 [Source:HGNC Symbol;Acc:HGNC:7980]
Entrez Summary This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Ensembl ID: ENSG00000123358
External Link: CisBP
Interpro IPR000536; IPR001628; IPR001723; IPR003070; IPR003071; IPR008946;
Protein Domain: ENSP00000243050
Protein Domain: ENSP00000378302
Protein Domain: ENSP00000449587
Protein Domain: ENSP00000353427
Protein Domain: ENSP00000440864
Protein Domain: ENSP00000457070
Protein Domain: ENSP00000456633
Protein Domain: ENSP00000456776
Protein Domain: ENSP00000455399
Protein: ENSP00000243050DBD: Nuclear Hormone ReceptorOther: Hormone_recep
Protein: ENSP00000378302DBD: Nuclear Hormone ReceptorOther: Hormone_recep
Protein: ENSP00000449587DBD: Nuclear Hormone ReceptorOther:
Protein: ENSP00000353427DBD: Nuclear Hormone ReceptorOther: Hormone_recep
Protein: ENSP00000440864DBD: Nuclear Hormone ReceptorOther: Hormone_recep
Protein: ENSP00000457070DBD: Nuclear Hormone ReceptorOther:
Protein: ENSP00000456633DBD: Nuclear Hormone ReceptorOther:
Protein: ENSP00000456776DBD: Nuclear Hormone ReceptorOther:
Protein: ENSP00000455399DBD: Nuclear Hormone ReceptorOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
ISS - GO_REF:0000024
ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity
IEA - GO_REF:0000002
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements


Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
SMiLE-seqIsakova2017Inferred - NR4A2 (92% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NR4A2 (92% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NR4A2 (92% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Nr4a2 (92% AA Identity, Mus musculus)
COMPILEDJASPARInferred - NR4A2 (92% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NR4A2 (92% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NR4A2 (92% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NR4A2 (92% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NR4A3 (91% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - Hr38 (85% AA Identity, Drosophila melanogaster)
PBMTF_EvolutionUnpublished DataInferred - nhr-6 (74% AA Identity, Caenorhabditis elegans)


Structure PDB 1CIT

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution