TF Info Page for BHLHE41 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: basic helix-loop-helix family member e41 [Source:HGNC Symbol;Acc:HGNC:16617]
Entrez Summary This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]
Ensembl ID: ENSG00000123095
External Link: CisBP
Interpro IPR003650; IPR011598;
Protein Domain: ENSP00000242728
Domain:
Protein: ENSP00000242728DBD: bHLHOther: Hairy_orange

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific
Transcription Factor Binding
tf co-factor binding_DNA Binding
Other_
PMIDS:12657651 15448136
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
b
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001078
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
ISS - GO_REF:0000024
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
ISS - GO_REF:0000024
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMZoo_01Inferred - Bhlhe41 (100% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - BHLHE40 (98% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHE40 (98% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - BHLHE40 (98% AA Identity, Homo sapiens)
SELEXJASPARInferred - BHLHE40 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - BHLHE40 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - BHLHE40 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Bhlhe40 (98% AA Identity, Mus musculus)
MiscHocoMocoInferred - BHLHE40 (98% AA Identity, Homo sapiens)
MiscHOMERInferred - BHLHE40 (98% AA Identity, Homo sapiens)
PBMBadis09Inferred - Bhlhe40 (96% AA Identity, Mus musculus)
PBMZoo_01Inferred - Bhlhe40 (96% AA Identity, Mus musculus)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution