TF Info Page for TWIST1 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Can form both homodimers and heterodimers with TCF3 (PMID: 16502419).

Description

Description: twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428]
Entrez Summary This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]
Ensembl ID: ENSG00000122691
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000242261
Domain:
Protein: ENSP00000242261DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:12210745
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IDA - PMID:17332325
GO:0043433
negative regulation of sequence-specific DNA binding transcription factor activity
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements Obligate_Multimer

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMZoo_01Inferred - Twist2 (96% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - twi (86% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - twi (86% AA Identity, Drosophila melanogaster)
DNaseI footprintingJASPARInferred - twi (86% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - twi (86% AA Identity, Drosophila melanogaster)
MisciDMMPMMInferred - twi (86% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - HAND2 (51% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HAND2 (51% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - BHLHA15 (50% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - BHLHA15 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHA15 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHA15 (50% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Bhlha15 (50% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - Fer1 (50% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution