TF Info Page for NFYB (Unknown)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 2 Obligate heteromer In vivo/Misc source Obligate heterotrimer of NFYA, NFYB, and NFYC

Description

Description: nuclear transcription factor Y subunit beta [Source:HGNC Symbol;Acc:HGNC:7805]
Entrez Summary The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000120837
External Link: CisBP
Interpro IPR003956; IPR003958; IPR009072; ;
Protein Domain: ENSP00000240055
Protein Domain: ENSP00000448250
Protein Domain: ENSP00000447486
Domain:
Protein: ENSP00000240055DBD: OtherOther: CBFD_NFYB_HMF, CENP-T_C, Histone, TFIID-18kDa
Protein: ENSP00000448250DBD: OtherOther: CBFD_NFYB_HMF, CENP-T_C, Histone, TFIID-18kDa
Protein: ENSP00000447486DBD: OtherOther: CBFD_NFYB_HMF, CENP-T_C, Histone, TFIID-18kDa

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Basal Transcription Factor_TF PPI_
PMIDS:12401788
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements Obligate_Multimer

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
ChIP-seqJASPARDirect
MiscHocoMocoDirect

Structure

Structure PDB 4AWL

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution