The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	E74 like ETS transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:3316]
Entrez Summary	TBA
Ensembl ID:	ENSG00000120690
External Link:	T077988_1.02
Interpro	IPR000418; IPR022084;
Protein Domain:
Protein: ENSP00000239882	DBD: ETS	Other: Elf-1_N
Protein: ENSP00000405580	DBD: ETS	Other:
Protein: ENSP00000384135	DBD: ETS	Other: Elf-1_N
Protein: ENSP00000486912	DBD: ETS	Other: Elf-1_N

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding TF PPI_ PMIDS:12065417 16002702
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IDA - PMID:8756667 GO:0003700 sequence-specific DNA binding transcription factor activity IDA - PMID:14970218
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2013		Direct
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
SELEX	JASPAR		Direct
ChIP-seq	modENCODE		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
Misc	HOMER		Direct
HT-SELEX	Yin2017		Inferred - ELF2 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF2 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF2 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF2 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF2 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF2 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF2 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF2 (95% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Elf2 (95% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - ELF4 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF4 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF4 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF4 (95% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Elf4 (95% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - ELF2 (95% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - ELF2 (95% AA Identity, Homo sapiens)
HT-SELEX	Nitta2015		Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)
SELEX	JASPAR		Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)
Transfac	Transfac	License required	Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)
Misc	iDMMPMM		Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 290, "endStyle": "curved", "aliStart": 210, "text": "Ets", "colour": "#009900", "aliEnd": 289, "start": 209, "href": "http://pfam.xfam.org/family/PF00178.20", "type": "pfama", "display": "true", "metadata": {"end": 290, "description": "The ETS domain is a winged helix-turn-helix structure that binds to DNA sites with a central GGA(A/T) DNA sequence. ", "database": "PfamA", "aliStart": 210, "scoreName": "E-value", "accession": "PF00178.20", "start": 209, "score": 1.4999999999999998e-34, "identifier": "Ets-domain", "type": "DBD", "aliEnd": 289}}, {"startStyle": "straight", "end": 111, "endStyle": "straight", "aliStart": 2, "text": "Elf-1_N", "colour": "#9999ff", "aliEnd": 111, "start": 2, "href": "http://pfam.xfam.org/family/PF12310.6", "type": "pfama", "display": "true", "metadata": {"end": 111, "description": "This domain family is found in eukaryotes, and is approximately 110 amino acids in length. The family is found in association with Pfam:PF00178. There is a conserved PAVIVE sequence motif. Elf-1 is an immune cell specific transcription factor. It is found in T cells, B cells, megakaryocytes,and mast cells and is involved in the control of transcription for various immune proteins. These include IL-2, GM-CSF, IL-5, IL-2 receptor alpha chain, and CD4 in T cells, IgH, blk, and lyn in B cells, TdT in T and B cells, IL-3 in megakaryocytes, and SCL and Fc-epsilon-RI alpha chain in mast cells.", "database": "PfamA", "aliStart": 2, "scoreName": "E-value", "accession": "PF12310.6", "start": 2, "score": 5.7e-45, "identifier": "Transcription factor protein N terminal", "type": "DBD", "aliEnd": 111}}], "length": 620}

{"regions": [{"startStyle": "straight", "end": 24, "endStyle": "jagged", "aliStart": 2, "text": "Elf-1_N", "colour": "#9999ff", "aliEnd": 24, "start": 2, "href": "http://pfam.xfam.org/family/PF12310.6", "type": "pfama", "display": "true", "metadata": {"end": 24, "description": "This domain family is found in eukaryotes, and is approximately 110 amino acids in length. The family is found in association with Pfam:PF00178. There is a conserved PAVIVE sequence motif. Elf-1 is an immune cell specific transcription factor. It is found in T cells, B cells, megakaryocytes,and mast cells and is involved in the control of transcription for various immune proteins. These include IL-2, GM-CSF, IL-5, IL-2 receptor alpha chain, and CD4 in T cells, IgH, blk, and lyn in B cells, TdT in T and B cells, IL-3 in megakaryocytes, and SCL and Fc-epsilon-RI alpha chain in mast cells.", "database": "PfamA", "aliStart": 2, "scoreName": "E-value", "accession": "PF12310.6", "start": 2, "score": 4.4e-07, "identifier": "Transcription factor protein N terminal", "type": "DBD", "aliEnd": 24}}], "length": 24}

{"regions": [{"startStyle": "curved", "end": 266, "endStyle": "curved", "aliStart": 186, "text": "Ets", "colour": "#009900", "aliEnd": 265, "start": 185, "href": "http://pfam.xfam.org/family/PF00178.20", "type": "pfama", "display": "true", "metadata": {"end": 266, "description": "The ETS domain is a winged helix-turn-helix structure that binds to DNA sites with a central GGA(A/T) DNA sequence. ", "database": "PfamA", "aliStart": 186, "scoreName": "E-value", "accession": "PF00178.20", "start": 185, "score": 1.3999999999999998e-34, "identifier": "Ets-domain", "type": "DBD", "aliEnd": 265}}, {"startStyle": "straight", "end": 111, "endStyle": "straight", "aliStart": 2, "text": "Elf-1_N", "colour": "#9999ff", "aliEnd": 111, "start": 2, "href": "http://pfam.xfam.org/family/PF12310.6", "type": "pfama", "display": "true", "metadata": {"end": 111, "description": "This domain family is found in eukaryotes, and is approximately 110 amino acids in length. The family is found in association with Pfam:PF00178. There is a conserved PAVIVE sequence motif. Elf-1 is an immune cell specific transcription factor. It is found in T cells, B cells, megakaryocytes,and mast cells and is involved in the control of transcription for various immune proteins. These include IL-2, GM-CSF, IL-5, IL-2 receptor alpha chain, and CD4 in T cells, IgH, blk, and lyn in B cells, TdT in T and B cells, IL-3 in megakaryocytes, and SCL and Fc-epsilon-RI alpha chain in mast cells.", "database": "PfamA", "aliStart": 2, "scoreName": "E-value", "accession": "PF12310.6", "start": 2, "score": 5.4e-45, "identifier": "Transcription factor protein N terminal", "type": "DBD", "aliEnd": 111}}], "length": 596}

TF Info Page for ELF1 (Ets)