TF Info Page for FOXO3 (Forkhead)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: forkhead box O3 [Source:HGNC Symbol;Acc:HGNC:3821]
Entrez Summary This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000118689
External Link: CisBP
Interpro IPR001766; ;
Protein Domain: ENSP00000339527
Protein Domain: ENSP00000385824
Protein Domain: ENSP00000446316
Domain:
Protein: ENSP00000339527DBD: ForkheadOther: FOXO-TAD, FOXO_KIX_bdg
Protein: ENSP00000385824DBD: ForkheadOther: FOXO-TAD, FOXO_KIX_bdg
Protein: ENSP00000446316DBD: ForkheadOther: FOXO-TAD, FOXO_KIX_bdg

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:10102273
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
b
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001227
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IDA - PMID:20371612
GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:14734530
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMDREAM_contestInferred - Foxo3 (100% AA Identity, Mus musculus)
PBMDREAM_contestInferred - Foxo1 (86% AA Identity, Mus musculus)
PBMDREAM_contestInferred - Foxo6 (86% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - FOXO1 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - FOXO1 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - FOXO1 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - FOXO1 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - FOXO1 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - FOXO1 (86% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - Foxo1 (86% AA Identity, Mus musculus)
MiscHocoMocoInferred - FOXO1 (86% AA Identity, Homo sapiens)
MiscHOMERInferred - Foxo1 (86% AA Identity, Mus musculus)
PBMDREAM_contestInferred - Foxo4 (82% AA Identity, Mus musculus)
PBMZoo_01Inferred - daf-16 (75% AA Identity, Caenorhabditis elegans)
HT-SELEXYin2017Inferred - FOXO4 (75% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - FOXO4 (75% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - FOXO4 (75% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - FOXO4 (75% AA Identity, Homo sapiens)
MiscHocoMocoInferred - FOXO4 (75% AA Identity, Homo sapiens)

Structure

Structure PDB 2UZK

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution