TF Info Page for MYB (Myb/SANT)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Contains 3 Myb DBDs

Description

Description: MYB proto-oncogene, transcription factor [Source:HGNC Symbol;Acc:HGNC:7545]
Entrez Summary This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Ensembl ID: ENSG00000118513
External Link: CisBP
Interpro IPR001005; IPR009057; IPR012642; IPR015395; IPR017877; IPR017930;
Protein Domain: ENSP00000339992
Protein Domain: ENSP00000356788
Protein Domain: ENSP00000410825
Protein Domain: ENSP00000435938
Protein Domain: ENSP00000434723
Protein Domain: ENSP00000436605
Protein Domain: ENSP00000435055
Protein Domain: ENSP00000432851
Domain:
Protein: ENSP00000339992DBD: Myb, Myb-Like, and SANT Domain Containing GenesOther: Cmyb_C, LMSTEN
Protein: ENSP00000356788DBD: Myb, Myb-Like, and SANT Domain Containing GenesOther: Cmyb_C, LMSTEN
Protein: ENSP00000410825DBD: Myb, Myb-Like, and SANT Domain Containing GenesOther: Cmyb_C, LMSTEN
Protein: ENSP00000435938DBD: Myb, Myb-Like, and SANT Domain Containing GenesOther: Cmyb_C, LMSTEN
Protein: ENSP00000434723DBD: Myb, Myb-Like, and SANT Domain Containing GenesOther: Cmyb_C, LMSTEN
Protein: ENSP00000436605DBD: Myb, Myb-Like, and SANT Domain Containing GenesOther: Cmyb_C
Protein: ENSP00000435055DBD: Myb, Myb-Like, and SANT Domain Containing GenesOther: Cmyb_C, LMSTEN
Protein: ENSP00000432851DBD: Myb, Myb-Like, and SANT Domain Containing GenesOther: Cmyb_C, LMSTEN

Previous Annotations

Source Annotation
TF-CAT classification TF Gene Candidate_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:12244173 15691758
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IC - GO_REF:0000036
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - Myb (78% AA Identity, Mus musculus)
ChIP-seqJASPARInferred - Myb (78% AA Identity, Mus musculus)
MiscHOMERInferred - Myb (78% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - MYB_AVIMB (75% AA Identity, Avian myeloblastosis virus)
TransfacTransfacLicense requiredInferred - MYB_AVIMB (75% AA Identity, Avian myeloblastosis virus)
PBMBadis09Inferred - Mybl1 (72% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - MYBL2 (72% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MYBL2 (72% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Mybl2 (72% AA Identity, Mus musculus)
MiscHocoMocoInferred - MYBL2 (72% AA Identity, Homo sapiens)
MiscHOMERInferred - MYBL2 (72% AA Identity, Homo sapiens)

Structure

Structure PDB 1MSE

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution