TF Info Page for CREB1 (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: cAMP responsive element binding protein 1 [Source:HGNC Symbol;Acc:HGNC:2345]
Entrez Summary This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
Ensembl ID: ENSG00000118260
External Link: CisBP
Interpro IPR001630; IPR003102; IPR004827; ;
Protein Domain: ENSP00000236995
Protein Domain: ENSP00000387699
Protein Domain: ENSP00000401803
Protein Domain: ENSP00000391125
Domain:
Protein: ENSP00000236995DBD: bZIPOther: pKID
Protein: ENSP00000387699DBD: bZIPOther: pKID
Protein: ENSP00000401803DBD: bZIPOther:
Protein: ENSP00000391125DBD: bZIPOther: pKID

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:14593102 15054091
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IC - GO_REF:0000036
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:8798441
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
IDA - PMID:19861239
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
COMPILEDJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMMann2013Inferred - Creb1 (100% AA Identity, Mus musculus)
PBMMann2013Inferred - Creb1 (100% AA Identity, Mus musculus)
PBMZoo_01Inferred - Creb1 (100% AA Identity, Mus musculus)
PBMZoo_01Inferred - Crem (95% AA Identity, Mus musculus)
PBMBadis09Inferred - Atf1 (86% AA Identity, Mus musculus)
PBMZoo_01Inferred - Atf1 (86% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - ATF1 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ATF1 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - ATF1 (86% AA Identity, Homo sapiens)
MiscHocoMocoInferred - ATF1 (86% AA Identity, Homo sapiens)
MiscHOMERInferred - ATF1 (86% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - CrebB-17A (77% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - CrebB-17A (77% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - CREM (75% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - CREM (75% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - CREM (75% AA Identity, Homo sapiens)
MiscHocoMocoInferred - CREM (75% AA Identity, Homo sapiens)

Structure

Structure PDB 1DH3

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution