TF Info Page for NR5A2 (Nuclear receptor)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: nuclear receptor subfamily 5 group A member 2 [Source:HGNC Symbol;Acc:HGNC:7984]
Entrez Summary The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development. [provided by RefSeq, Jun 2016]
Ensembl ID: ENSG00000116833
External Link: CisBP
Interpro IPR000536; IPR001628; IPR001723; IPR008946; IPR016355; ;
Protein Domain: ENSP00000236914
Protein Domain: ENSP00000356326
Protein Domain: ENSP00000356331
Protein Domain: ENSP00000414888
Protein Domain: ENSP00000439116
Domain:
Protein: ENSP00000236914DBD: Nuclear Hormone ReceptorOther: Hormone_recep
Protein: ENSP00000356326DBD: Nuclear Hormone ReceptorOther:
Protein: ENSP00000356331DBD: Nuclear Hormone ReceptorOther: Hormone_recep
Protein: ENSP00000414888DBD: Nuclear Hormone ReceptorOther:
Protein: ENSP00000439116DBD: Nuclear Hormone ReceptorOther: Hormone_recep

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:12456679 15014077
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:15205472, PMID:15723037, PMID:16289203
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
TAS - PMID:9786908
GO:0004879
ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity
TAS - PMID:16271724
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMDREAM_contestInferred - Nr5a2 (94% AA Identity, Mus musculus)
ChIP-seqJASPARInferred - Nr5a2 (94% AA Identity, Mus musculus)
MiscHOMERInferred - Nr5a2 (94% AA Identity, Mus musculus)
MiscHOMERInferred - Nr5a2 (94% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - NR5A1 (88% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NR5A1 (88% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NR5A1 (88% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NR5A1 (88% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NR5A1 (88% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NR5A1 (88% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NR5A1 (88% AA Identity, Homo sapiens)
PBMZoo_01Inferred - ftz-f1 (87% AA Identity, Drosophila melanogaster)
ChIP-seqmodENCODEInferred - nhr-25 (75% AA Identity, Caenorhabditis elegans)

Structure

Structure PDB 2A66

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution