TF Info Page for NFE2L2 (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 2 Obligate heteromer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Has been tested by both PBM and HT-SELEX. Neither yielded a motif. Likely obligate heteromer.

Description

Description: nuclear factor, erythroid 2 like 2 [Source:HGNC Symbol;Acc:HGNC:7782]
Entrez Summary NA
Ensembl ID: ENSG00000116044
External Link: CisBP
Interpro IPR004826; IPR004827; IPR008917; ;
Protein Domain: ENSP00000380252
Protein Domain: ENSP00000380253
Protein Domain: ENSP00000412191
Protein Domain: ENSP00000410015
Protein Domain: ENSP00000411575
Protein Domain: ENSP00000400073
Protein Domain: ENSP00000467401
Protein Domain: ENSP00000391291
Protein Domain: ENSP00000416308
Domain:
Protein: ENSP00000380252DBD: bZIPOther:
Protein: ENSP00000380253DBD: bZIPOther:
Protein: ENSP00000412191DBD: bZIPOther:
Protein: ENSP00000410015DBD: bZIPOther:
Protein: ENSP00000411575DBD: bZIPOther:
Protein: ENSP00000400073DBD: bZIPOther:
Protein: ENSP00000467401DBD: bZIPOther:
Protein: ENSP00000391291DBD: bZIPOther:
Protein: ENSP00000416308DBD: bZIPOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific__
PMIDS:0
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001205
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involv
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:17015834
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements Obligate_Multimer

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
ChIP-seqJASPARInferred - Nfe2l2 (88% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - NFE2 (63% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFE2 (63% AA Identity, Homo sapiens)
PBMTF_EvolutionUnpublished DataInferred - NFE2L2 (63% AA Identity, Gallus gallus)
ChIP-seqENCODEInferred - NFE2 (63% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (63% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (63% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (63% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NFE2 (63% AA Identity, Homo sapiens)
MiscHOMERInferred - NFE2 (63% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution