TF Info Page for NFE2L2 (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 2 Obligate heteromer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Has been tested by both PBM and HT-SELEX. Neither yielded a motif. Likely obligate heteromer.

Description

Description: nuclear factor, erythroid 2 like 2 [Source:HGNC Symbol;Acc:HGNC:7782]
Entrez Summary This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]
Ensembl ID: ENSG00000116044
External Link: CisBP
Interpro IPR004826; IPR004827; IPR008917; ;
Protein Domain: ENSP00000380252
Protein Domain: ENSP00000380253
Protein Domain: ENSP00000412191
Protein Domain: ENSP00000410015
Protein Domain: ENSP00000411575
Protein Domain: ENSP00000400073
Protein Domain: ENSP00000467401
Protein Domain: ENSP00000391291
Protein Domain: ENSP00000416308
Domain:
Protein: ENSP00000380252DBD: bZIPOther:
Protein: ENSP00000380253DBD: bZIPOther:
Protein: ENSP00000412191DBD: bZIPOther:
Protein: ENSP00000410015DBD: bZIPOther:
Protein: ENSP00000411575DBD: bZIPOther:
Protein: ENSP00000400073DBD: bZIPOther:
Protein: ENSP00000467401DBD: bZIPOther:
Protein: ENSP00000391291DBD: bZIPOther:
Protein: ENSP00000416308DBD: bZIPOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific__
PMIDS:0
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001205
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involv
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:17015834
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements Obligate_Multimer

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
ChIP-seqJASPARInferred - Nfe2l2 (88% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - NFE2 (63% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFE2 (63% AA Identity, Homo sapiens)
PBMTF_EvolutionUnpublished DataInferred - NFE2L2 (63% AA Identity, Gallus gallus)
ChIP-seqENCODEInferred - NFE2 (63% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (63% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (63% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (63% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NFE2 (63% AA Identity, Homo sapiens)
MiscHOMERInferred - NFE2 (63% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution