TF Info Page for EPAS1 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain

Description

Description: endothelial PAS domain protein 1 [Source:HGNC Symbol;Acc:HGNC:3374]
Entrez Summary This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
Ensembl ID: ENSG00000116016
External Link: CisBP
Interpro IPR000014; IPR001067; IPR001610; IPR011598; IPR013767; IPR014887; IPR021537; ;
Protein Domain: ENSP00000263734
Protein Domain: ENSP00000406137
Domain:
Protein: ENSP00000263734DBD: bHLHOther: HIF-1, HIF-1a_CTAD, PAS, PAS_11, PAS_3, PAS_4, PAS
Protein: ENSP00000406137DBD: bHLHOther: PAS, PAS_11, PAS_3, PAS_4, PAS_9

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_Transcription Factor Binding
tf co-factor binding
DNA-Binding
sequence-specific_Co-activation
DNA Binding_
PMIDS:15190133
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements Obligate_Multimer

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERDirect
TransfacTransfacLicense requiredInferred - HIF1A (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - HIF1A (81% AA Identity, Homo sapiens)
MiscHocoMocoInferred - HIF1A (81% AA Identity, Homo sapiens)
MiscHOMERInferred - HIF1A (81% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Hif3a (74% AA Identity, Mus musculus)

Structure

Structure PDB 4ZPK

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution