TF Info Page for ATF2 (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: activating transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:784]
Entrez Summary This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]
Ensembl ID: ENSG00000115966
External Link: CisBP
Interpro IPR004827; IPR007087; IPR016378; ;
Protein Domain: ENSP00000264110
Protein Domain: ENSP00000340576
Protein Domain: ENSP00000376327
Protein Domain: ENSP00000386282
Protein Domain: ENSP00000386526
Protein Domain: ENSP00000407911
Domain:
Protein: ENSP00000264110DBD: bZIPOther:
Protein: ENSP00000340576DBD: bZIPOther:
Protein: ENSP00000376327DBD: bZIPOther:
Protein: ENSP00000386282DBD: bZIPOther:
Protein: ENSP00000386526DBD: bZIPOther:
Protein: ENSP00000407911DBD: bZIPOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_Transactivation_
PMIDS:1658708 2529117 9139739 10909971
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IC - GO_REF:0000036
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:8798441
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
IC - PMID:19861239
GO:0051090
regulation of sequence-specific DNA binding transcription factor activity
TAS - Reactome:R-HSA-450341
GO:0051091
positive regulation of sequence-specific DNA binding transcription factor activity
IMP - PMID:10821277
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
PBMZoo_01Inferred - Atf2 (100% AA Identity, Mus musculus)
MiscHOMERInferred - Atf2 (100% AA Identity, Mus musculus)
PBMZoo_01Inferred - Atf7 (82% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - CREB5 (81% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - CREB5 (81% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - CREB5 (81% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - CREB5 (81% AA Identity, Homo sapiens)

Structure

Structure PDB 1T2K

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution