TF Info Page for HAND1 (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 2 Obligate heteromer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Obligate heteromer (PMID: 10611232).

Description

Description: heart and neural crest derivatives expressed 1 [Source:HGNC Symbol;Acc:HGNC:4807]
Entrez Summary The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000113196
External Link: CisBP
Interpro IPR011598; ;
Protein Domain: ENSP00000231121
Domain:
Protein: ENSP00000231121DBD: bHLHOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:7791788 11802795 16043483 7671815 15509787 16043483 10611232 16043483
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001078
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:11802795
GO:0043433
negative regulation of sequence-specific DNA binding transcription factor activity
IDA - PMID:11802795
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
MiscHocoMocoDirect
HT-SELEXYin2017Inferred - HAND2 (88% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - HAND2 (88% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - BHLHA15 (50% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - BHLHA15 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHA15 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - BHLHA15 (50% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Bhlha15 (50% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - NHLH1 (50% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NHLH1 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NHLH1 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NHLH1 (50% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - NHLH2 (50% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NHLH2 (50% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Nhlh2 (50% AA Identity, Mus musculus)
SELEXJASPARInferred - NHLH1 (50% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NHLH1 (50% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NHLH1 (50% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Nhlh2 (50% AA Identity, Mus musculus)
MiscHocoMocoInferred - NHLH1 (50% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution