The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

transcription factor EB [Source:HGNC Symbol;Acc:HGNC:11753]

Entrez Summary

Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including cellular response to amino acid starvation; lysosome localization; and positive regulation of autophagy. Located in cytosol; lysosomal membrane; and nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022]

Ensembl ID:

ENSG00000112561

External Link:

CisBP

Interpro

IPR011598; IPR021802;

Protein Domain:

ENSP00000230323

Protein Domain:

ENSP00000351742

Protein Domain:

ENSP00000362124

Protein Domain:

ENSP00000383998

Protein Domain:

ENSP00000396168

Protein Domain:

ENSP00000415035

Protein Domain:

ENSP00000415946

Protein Domain:

ENSP00000395208

Protein Domain:

ENSP00000343948

Protein Domain:

ENSP00000377824

Protein Domain:

ENSP00000400276

Domain:

Protein: ENSP00000230323	DBD: bHLH	Other: DUF3371, MITF_TFEB_C_3_N
Protein: ENSP00000351742	DBD: bHLH	Other: DUF3371, MITF_TFEB_C_3_N
Protein: ENSP00000362124	DBD: bHLH	Other: DUF3371, MITF_TFEB_C_3_N
Protein: ENSP00000383998	DBD: bHLH	Other: DUF3371
Protein: ENSP00000396168	DBD: bHLH	Other: MITF_TFEB_C_3_N
Protein: ENSP00000415035	DBD: bHLH	Other: MITF_TFEB_C_3_N
Protein: ENSP00000415946	DBD: bHLH	Other:
Protein: ENSP00000395208	DBD: bHLH	Other:
Protein: ENSP00000343948	DBD: bHLH	Other: DUF3371, MITF_TFEB_C_3_N
Protein: ENSP00000377824	DBD: bHLH	Other: MITF_TFEB_C_3_N
Protein: ENSP00000400276	DBD: bHLH	Other: MITF_TFEB_C_3_N

Previous Annotations

Source	Annotation
TF-CAT classification	Indeterminate - There is no evidence for or against this genes role as a TF___ PMIDS:0
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0003700 sequence-specific DNA binding transcription factor activity IDA - PMID:19556463 \| NAS - PMID:2115126
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2010		Direct
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Misc	HocoMoco		Direct
PBM	Zoo_01		Inferred - Tfeb (100% AA Identity, Mus musculus)
SMiLE-seq	Isakova2017		Inferred - TFE3 (87% AA Identity, Mus musculus)
PBM	Zoo_01		Inferred - TFE3 (87% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - TFE3 (87% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - TFE3 (85% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - TFE3 (85% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - TFE3 (85% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - TFE3 (85% AA Identity, Homo sapiens)
PBM	DREAM_contest		Inferred - Tfec (78% AA Identity, Mus musculus)
PBM	Zoo_01		Inferred - Tfec (78% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - TFEC (76% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - TFEC (76% AA Identity, Homo sapiens)
PBM	Grove09		Inferred - hlh-30 (70% AA Identity, Caenorhabditis elegans)
PBM	Zoo_01		Inferred - Mitf (55% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - MITF (55% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - MITF (55% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - MITF (55% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - Mitf (55% AA Identity, Mus musculus)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for TFEB (bHLH)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution