TF Info Page for E2F3 (E2F)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro Binds preferentially with DP proteins in vivo

Description

Description: E2F transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:3115]
Entrez Summary This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Ensembl ID: ENSG00000112242
External Link: CisBP
Interpro IPR003316; ;
Protein Domain: ENSP00000262904
Protein Domain: ENSP00000443418
Protein Domain: ENSP00000483249
Domain:
Protein: ENSP00000262904DBD: E2FOther: E2F_CC-MB
Protein: ENSP00000443418DBD: E2FOther: E2F_CC-MB
Protein: ENSP00000483249DBD: E2FOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene Candidate_DNA-Binding
sequence-specific_Co-activation_
PMIDS:15448153 17135268
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
SELEXJASPARDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - E2f3 (100% AA Identity, Mus musculus)
PBMZoo_01Inferred - E2F3 (96% AA Identity, Taeniopygia guttata)
PBMZoo_01Inferred - ENSOANG00000014967 (86% AA Identity, Ornithorhynchus anatinus)
HT-SELEXYin2017Inferred - E2F2 (84% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - E2F2 (84% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - E2F2 (84% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - E2F2 (84% AA Identity, Homo sapiens)
PBMZoo_01Inferred - E2F2 (84% AA Identity, Homo sapiens)
MiscHocoMocoInferred - E2F2 (84% AA Identity, Homo sapiens)
PBMZoo_01Inferred - E2F2 (83% AA Identity, Tupaia belangeri)
HT-SELEXYin2017Inferred - E2F1 (76% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - E2F1 (76% AA Identity, Homo sapiens)
SELEXJASPARInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (76% AA Identity, Homo sapiens)
MiscHocoMocoInferred - E2F1 (76% AA Identity, Homo sapiens)
MiscHOMERInferred - E2F1 (76% AA Identity, Homo sapiens)
PBMZoo_01Inferred - e_gw1.38.2.1 (75% AA Identity, Physcomitrella patens)
PBMTF_EvolutionUnpublished DataInferred - estExt_gwp_gw1.C_220046 (75% AA Identity, Physcomitrella patens)
Dap-seqOMalley2016Inferred - E2FA (72% AA Identity, Arabidopsis thaliana)
Dap-seqOMalley2016Inferred - E2FA (72% AA Identity, Arabidopsis thaliana)
ChIP-seqmodENCODEInferred - E2f (70% AA Identity, Drosophila melanogaster)
PBMBadis09Inferred - E2f2 (69% AA Identity, Mus musculus)
PBMZoo_01Inferred - E2F2 (67% AA Identity, Anolis carolinensis)
Dap-seqOMalley2016Inferred - E2FC (67% AA Identity, Arabidopsis thaliana)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution