TF Info Page for BACH2 (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro Based on Newman et al 2003 (PMID: 12805554), the protein has strong preference for forming heterodimers with MAFG and MAFK over homo-dimerisation. The Homer ChIP-seq motif appears to be a MAF-BACH2 heterodimer.

Description

Description: BTB domain and CNC homolog 2 [Source:HGNC Symbol;Acc:HGNC:14078]
Entrez Summary NA
Ensembl ID: ENSG00000112182
External Link: CisBP
Interpro IPR000210; IPR004826; IPR004827; IPR008917; IPR011333; IPR013069;
Protein Domain: ENSP00000257749
Protein Domain: ENSP00000437473
Protein Domain: ENSP00000384145
Domain:
Protein: ENSP00000257749DBD: bZIPOther: BTB
Protein: ENSP00000437473DBD: bZIPOther: BTB
Protein: ENSP00000384145DBD: bZIPOther: BTB

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:8887638 10949928
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001206
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involv
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
MiscHOMERDirect
ChIP-seqmodENCODEInferred - BACH1 (68% AA Identity, Homo sapiens)
ChIP-seqmodENCODEInferred - BACH1 (68% AA Identity, Homo sapiens)
ChIP-seqmodENCODEInferred - BACH1 (68% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - BACH1 (68% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - BACH1 (68% AA Identity, Homo sapiens)
MiscHocoMocoInferred - BACH1 (68% AA Identity, Homo sapiens)
MiscHOMERInferred - BACH1 (68% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution