The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	E74 like ETS transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:3317]
Entrez Summary	TBA
Ensembl ID:	ENSG00000109381
External Link:
Interpro	IPR000418; IPR022084;
Protein Domain:
Protein: ENSP00000351458	DBD: ETS	Other: Elf-1_N
Protein: ENSP00000397796	DBD: ETS	Other: Elf-1_N
Protein: ENSP00000426997	DBD: ETS	Other: Elf-1_N
Protein: ENSP00000368867	DBD: ETS	Other: Elf-1_N
Protein: ENSP00000426087	DBD: ETS	Other: Elf-1_N

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding Transactivation_ PMIDS:8756667
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0000981 sequence-specific DNA binding RNA polymerase II transcription factor activity IBA - GO_REF:0000033
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
PBM	Wei10		Inferred - Elf2 (100% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - ELF1 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF1 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF1 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF1 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF1 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF1 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF1 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF1 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF4 (95% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - ELF4 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF4 (95% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - ELF4 (95% AA Identity, Homo sapiens)
PBM	Wei10		Inferred - Elf4 (95% AA Identity, Mus musculus)
ChIP-seq	ENCODE		Inferred - ELF1 (95% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - ELF1 (95% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - ELF1 (95% AA Identity, Homo sapiens)
SELEX	JASPAR		Inferred - ELF1 (95% AA Identity, Homo sapiens)
ChIP-seq	modENCODE		Inferred - ELF1 (95% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELF1 (95% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELF1 (95% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELF1 (95% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELF1 (95% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - ELF1 (95% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - ELF1 (95% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - ELF1 (95% AA Identity, Homo sapiens)
HT-SELEX	Nitta2015		Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)
SELEX	JASPAR		Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)
Transfac	Transfac	License required	Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)
Misc	iDMMPMM		Inferred - Eip74EF (86% AA Identity, Drosophila melanogaster)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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{"regions": [{"startStyle": "curved", "end": 218, "endStyle": "curved", "aliStart": 138, "text": "Ets", "colour": "#009900", "aliEnd": 217, "start": 137, "href": "http://pfam.xfam.org/family/PF00178.20", "type": "pfama", "display": "true", "metadata": {"end": 218, "description": "The ETS domain is a winged helix-turn-helix structure that binds to DNA sites with a central GGA(A/T) DNA sequence. ", "database": "PfamA", "aliStart": 138, "scoreName": "E-value", "accession": "PF00178.20", "start": 137, "score": 1.2e-34, "identifier": "Ets-domain", "type": "DBD", "aliEnd": 217}}, {"startStyle": "jagged", "end": 48, "endStyle": "straight", "aliStart": 20, "text": "Elf-1_N", "colour": "#9999ff", "aliEnd": 48, "start": 9, "href": "http://pfam.xfam.org/family/PF12310.6", "type": "pfama", "display": "true", "metadata": {"end": 48, "description": "This domain family is found in eukaryotes, and is approximately 110 amino acids in length. The family is found in association with Pfam:PF00178. There is a conserved PAVIVE sequence motif. Elf-1 is an immune cell specific transcription factor. It is found in T cells, B cells, megakaryocytes,and mast cells and is involved in the control of transcription for various immune proteins. These include IL-2, GM-CSF, IL-5, IL-2 receptor alpha chain, and CD4 in T cells, IgH, blk, and lyn in B cells, TdT in T and B cells, IL-3 in megakaryocytes, and SCL and Fc-epsilon-RI alpha chain in mast cells.", "database": "PfamA", "aliStart": 20, "scoreName": "E-value", "accession": "PF12310.6", "start": 9, "score": 5.3e-06, "identifier": "Transcription factor protein N terminal", "type": "DBD", "aliEnd": 48}}], "length": 522}

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{"regions": [{"startStyle": "curved", "end": 189, "endStyle": "curved", "aliStart": 109, "text": "Ets", "colour": "#009900", "aliEnd": 188, "start": 108, "href": "http://pfam.xfam.org/family/PF00178.20", "type": "pfama", "display": "true", "metadata": {"end": 189, "description": "The ETS domain is a winged helix-turn-helix structure that binds to DNA sites with a central GGA(A/T) DNA sequence. ", "database": "PfamA", "aliStart": 109, "scoreName": "E-value", "accession": "PF00178.20", "start": 108, "score": 2e-35, "identifier": "Ets-domain", "type": "DBD", "aliEnd": 188}}, {"startStyle": "jagged", "end": 48, "endStyle": "straight", "aliStart": 20, "text": "Elf-1_N", "colour": "#9999ff", "aliEnd": 48, "start": 7, "href": "http://pfam.xfam.org/family/PF12310.6", "type": "pfama", "display": "true", "metadata": {"end": 48, "description": "This domain family is found in eukaryotes, and is approximately 110 amino acids in length. The family is found in association with Pfam:PF00178. There is a conserved PAVIVE sequence motif. Elf-1 is an immune cell specific transcription factor. It is found in T cells, B cells, megakaryocytes,and mast cells and is involved in the control of transcription for various immune proteins. These include IL-2, GM-CSF, IL-5, IL-2 receptor alpha chain, and CD4 in T cells, IgH, blk, and lyn in B cells, TdT in T and B cells, IL-3 in megakaryocytes, and SCL and Fc-epsilon-RI alpha chain in mast cells.", "database": "PfamA", "aliStart": 20, "scoreName": "E-value", "accession": "PF12310.6", "start": 7, "score": 6.6e-07, "identifier": "Transcription factor protein N terminal", "type": "DBD", "aliEnd": 48}}], "length": 192}

TF Info Page for ELF2 (Ets)