TF Info Page for HLF (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: HLF, PAR bZIP transcription factor [Source:HGNC Symbol;Acc:HGNC:4977]
Entrez Summary This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000108924
External Link: CisBP
Interpro IPR004827; ;
Protein Domain: ENSP00000226067
Protein Domain: ENSP00000402496
Protein Domain: ENSP00000460572
Protein Domain: ENSP00000461455
Domain:
Protein: ENSP00000226067DBD: bZIPOther:
Protein: ENSP00000402496DBD: bZIPOther:
Protein: ENSP00000460572DBD: bZIPOther:
Protein: ENSP00000461455DBD: bZIPOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:8065331
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
b
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
SELEXJASPARDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
HT-SELEXYin2017Inferred - TEF (78% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - TEF (78% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Tef (78% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - TEF (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TEF (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TEF (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TEF (78% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TEF (78% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - DBP (65% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - DBP (65% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - DBP (65% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - DBP (65% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Dbp (65% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - DBP (65% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - DBP (65% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - DBP (65% AA Identity, Homo sapiens)
MiscHocoMocoInferred - DBP (65% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Dbp (64% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - Pdp1 (63% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - Pdp1 (63% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution