TF Info Page for GLIS3 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: GLIS family zinc finger 3 [Source:HGNC Symbol;Acc:HGNC:28510]
Entrez Summary This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000107249
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000325494
Protein Domain: ENSP00000418671
Protein Domain: ENSP00000417794
Protein Domain: ENSP00000418995
Protein Domain: ENSP00000418005
Protein Domain: ENSP00000417883
Domain:
Protein: ENSP00000325494DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000418671DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000417794DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000418995DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000418005DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000417883DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
b
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBusserPNAS2012Inferred - lmd (78% AA Identity, Drosophila melanogaster)
HT-SELEXNitta2015Inferred - lmd (78% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution