TF Info Page for GLI3 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: GLI family zinc finger 3 [Source:HGNC Symbol;Acc:HGNC:4319]
Entrez Summary This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000106571
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000407963
Protein Domain: ENSP00000406135
Protein Domain: ENSP00000379258
Domain:
Protein: ENSP00000407963DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000406135DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000379258DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:10433919 12435627 16396903
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IC - GO_REF:0000036
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:10077605, PMID:17000779, PMID:17764085
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
SELEXHallikas2006Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMPeterson2012Inferred - Gli3 (98% AA Identity, Mus musculus)
ChIP-seqhmCHIPInferred - Gli3 (98% AA Identity, Mus musculus)
MiscHOMERInferred - Gli3 (98% AA Identity, Mus musculus)
HT-SELEXJolma2010Inferred - GLI2 (92% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - GLI2 (92% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - GLI2 (92% AA Identity, Homo sapiens)
SELEXHallikas2006Inferred - GLI2 (92% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GLI2 (92% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GLI2 (92% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GLI2 (92% AA Identity, Homo sapiens)
MiscHocoMocoInferred - GLI2 (92% AA Identity, Homo sapiens)
PBMPeterson2012Inferred - Gli2 (91% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - ci (85% AA Identity, Drosophila melanogaster)
SELEXHallikas2006Inferred - ci (85% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - ci (85% AA Identity, Drosophila melanogaster)
SELEXHallikas2006Inferred - GLI1 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GLI1 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GLI1 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GLI1 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GLI1 (85% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - GLI1 (85% AA Identity, Homo sapiens)
MiscHocoMocoInferred - GLI1 (85% AA Identity, Homo sapiens)
PBMPeterson2012Inferred - Gli1 (84% AA Identity, Mus musculus)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution