TF Info Page for POU6F2 (Homeodomain; POU)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: POU class 6 homeobox 2 [Source:HGNC Symbol;Acc:HGNC:21694]
Entrez Summary This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Ensembl ID: ENSG00000106536
External Link: CisBP
Interpro IPR000327; IPR001356; IPR009057; IPR010982; IPR013847; ;
Protein Domain: ENSP00000430514
Protein Domain: ENSP00000409684
Domain:
Protein: ENSP00000430514DBD: Homeodomain,POUOther:
Protein: ENSP00000409684DBD: Homeodomain,POUOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:8601806
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
PBMBarrera2016Direct
PBMBarrera2016Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Inferred - POU6F1 (86% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU6F1 (86% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU6F1 (86% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU6F1 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU6F1 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU6F1 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU6F1 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU6F1 (86% AA Identity, Homo sapiens)
PBMBerger08Inferred - Pou6f1 (86% AA Identity, Mus musculus)
PBMBerger08Inferred - Pou6f1 (86% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - POU6F1 (86% AA Identity, Homo sapiens)
MiscHocoMocoInferred - POU6F1 (86% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution