TF Info Page for TFEC (bHLH)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: transcription factor EC [Source:HGNC Symbol;Acc:HGNC:11754]
Entrez Summary This gene encodes a member of the micropthalmia (MiT) family of basic helix-loop-helix leucine zipper transcription factors. MiT transcription factors regulate the expression of target genes by binding to E-box recognition sequences as homo- or heterodimers, and play roles in multiple cellular processes including survival, growth and differentiation. The encoded protein is a transcriptional activator of the nonmuscle myosin II heavy chain-A gene, and may also co-regulate target genes in osteoclasts as a heterodimer with micropthalmia-associated transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Ensembl ID: ENSG00000105967
External Link: CisBP
Interpro IPR011598; IPR021802;
Protein Domain: ENSP00000265440
Protein Domain: ENSP00000318676
Protein Domain: ENSP00000377125
Protein Domain: ENSP00000417432
Protein Domain: ENSP00000387650
Domain:
Protein: ENSP00000265440DBD: bHLHOther: DUF3371
Protein: ENSP00000318676DBD: bHLHOther: DUF3371
Protein: ENSP00000377125DBD: bHLHOther:
Protein: ENSP00000417432DBD: bHLHOther: MITF_TFEB_C_3_N
Protein: ENSP00000387650DBD: bHLHOther: DUF3371

Previous Annotations

Source Annotation
TF-CAT classification TF Gene Candidate_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:8336698
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:9256061
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
PBMDREAM_contestInferred - Tfec (98% AA Identity, Mus musculus)
PBMZoo_01Inferred - Tfec (98% AA Identity, Mus musculus)
PBMZoo_01Inferred - Mitf (83% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - MITF (83% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MITF (83% AA Identity, Homo sapiens)
MiscHocoMocoInferred - MITF (83% AA Identity, Homo sapiens)
MiscHOMERInferred - Mitf (83% AA Identity, Mus musculus)
SMiLE-seqIsakova2017Inferred - TFE3 (82% AA Identity, Mus musculus)
PBMZoo_01Inferred - TFE3 (82% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - TFE3 (82% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - TFE3 (80% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - TFE3 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TFE3 (80% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TFE3 (80% AA Identity, Homo sapiens)
HT-SELEXJolma2010Inferred - TFEB (76% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - TFEB (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - TFEB (76% AA Identity, Homo sapiens)
PBMZoo_01Inferred - Tfeb (76% AA Identity, Mus musculus)
MiscHocoMocoInferred - TFEB (76% AA Identity, Homo sapiens)
PBMGrove09Inferred - hlh-30 (67% AA Identity, Caenorhabditis elegans)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution