TF Info Page for SP4 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Sp4 transcription factor [Source:HGNC Symbol;Acc:HGNC:11209]
Entrez Summary The protein encoded by this gene is a transcription factor that can bind to the GC promoter region of a variety of genes, including those of the photoreceptor signal transduction system. The encoded protein binds to the same sites in promoter CpG islands as does the transcription factor SP1, although its expression is much more restricted compared to that of SP1. This gene may be involved in bipolar disorder and schizophrenia. [provided by RefSeq, May 2016]
Ensembl ID: ENSG00000105866
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000222584
Protein Domain: ENSP00000393623
Domain:
Protein: ENSP00000222584DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000393623DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:15265862
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
ChIP-seqSchmitges2016Direct
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - Sp4 (98% AA Identity, Mus musculus)
SMiLE-seqIsakova2017Inferred - Sp4 (98% AA Identity, Mus musculus)
PBMZoo_01Inferred - Sp4 (98% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - SP3 (91% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP3 (91% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP3 (91% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP3 (91% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Sp1 (89% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - SP1 (87% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP1 (87% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - SP8 (87% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP8 (87% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - SP1 (87% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - SP1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (87% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP1 (87% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP1 (87% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP1 (87% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - Sp1 (86% AA Identity, Drosophila melanogaster)
HT-SELEXYin2017Inferred - SP9 (86% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP9 (86% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP5 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP7 (80% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - SP2 (76% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - SP2 (76% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - SP2 (76% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - SP2 (76% AA Identity, Homo sapiens)
MiscHocoMocoInferred - SP2 (76% AA Identity, Homo sapiens)
PBMLam11Inferred - ZFA5_CONSTRUCT (75% AA Identity, PBM CONSTRUCTS)
PBMCEPDInferred - sptf-3 (72% AA Identity, Caenorhabditis elegans)
PBMLam11Inferred - ZFA93_CONSTRUCT (72% AA Identity, PBM CONSTRUCTS)
PBMLam11Inferred - ZFA4_CONSTRUCT (71% AA Identity, PBM CONSTRUCTS)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution