The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro	Has a putative AT-hook

Description

Description:

upstream transcription factor 2, c-fos interacting [Source:HGNC Symbol;Acc:HGNC:12594]

Entrez Summary

This gene encodes a member of the basic helix-loop-helix leucine zipper family of transcription factors. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs and is involved in regulating multiple cellular processes. [provided by RefSeq, Mar 2016]

Ensembl ID:

ENSG00000105698

External Link:

CisBP

Interpro

IPR011598; ;

Protein Domain:

ENSP00000222305

Protein Domain:

ENSP00000340633

Protein Domain:

ENSP00000368429

Protein Domain:

ENSP00000471511

Protein Domain:

ENSP00000469585

Protein Domain:

ENSP00000470081

Protein Domain:

ENSP00000471775

Protein Domain:

ENSP00000469364

Domain:

Protein: ENSP00000222305	DBD: bHLH	Other:
Protein: ENSP00000340633	DBD: bHLH	Other:
Protein: ENSP00000368429	DBD: bHLH	Other:
Protein: ENSP00000471511	DBD: bHLH	Other:
Protein: ENSP00000469585	DBD: bHLH	Other:
Protein: ENSP00000470081	DBD: bHLH	Other:
Protein: ENSP00000471775	DBD: bHLH	Other:
Protein: ENSP00000469364	DBD: bHLH	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:15242350
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0003700 sequence-specific DNA binding transcription factor activity TAS - PMID:1589769, PMID:8954795 GO:0003705 RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity IMP - PMID:8576131
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	ENCODE		Direct
ChIP-seq	JASPAR		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
Misc	HOMER		Inferred - Usf2 (98% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - USF1 (85% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - USF1 (85% AA Identity, Homo sapiens)
PBM	Zoo_01		Inferred - Usf1 (85% AA Identity, Mus musculus)
ChIP-seq	ENCODE		Inferred - USF1 (85% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - USF1 (85% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - USF1 (85% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - USF1 (85% AA Identity, Homo sapiens)
ChIP-seq	ENCODE		Inferred - USF1 (85% AA Identity, Homo sapiens)
ChIP-seq	JASPAR		Inferred - USF1 (85% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - USF1 (85% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - USF1 (85% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - USF1 (85% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - USF1 (85% AA Identity, Homo sapiens)
Misc	HOMER		Inferred - USF1 (85% AA Identity, Homo sapiens)
PBM	Zoo_01		Inferred - Usf2 (53% AA Identity, Mus musculus)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

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TF Info Page for USF2 (bHLH)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution