The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:

nuclear factor of activated T-cells 5, tonicity-responsive [Source:HGNC Symbol;Acc:HGNC:7774]

Entrez Summary

Ensembl ID:

ENSG00000102908

External Link:

CisBP

Interpro

IPR002909; IPR008366; IPR008967; IPR011539; IPR014756; ;

Protein Domain:

ENSP00000338806

Protein Domain:

ENSP00000346420

Protein Domain:

ENSP00000377343

Protein Domain:

ENSP00000455628

Protein Domain:

ENSP00000457593

Protein Domain:

ENSP00000463214

Protein Domain:

ENSP00000486610

Domain:

Protein: ENSP00000338806	DBD: RHD	Other: RHD_dimer
Protein: ENSP00000346420	DBD: RHD	Other: RHD_dimer
Protein: ENSP00000377343	DBD: RHD	Other: RHD_dimer
Protein: ENSP00000455628	DBD: RHD	Other: RHD_dimer
Protein: ENSP00000457593	DBD: RHD	Other: RHD_dimer
Protein: ENSP00000463214	DBD: RHD	Other:
Protein: ENSP00000486610	DBD: RHD	Other: RHD_dimer

Previous Annotations

Source	Annotation
TF-CAT classification	TF Gene_DNA-Binding sequence-specific_DNA Binding_ PMIDS:10377394 11485737 16027109
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a IDA - PMID:11485737 GO:0003700 sequence-specific DNA binding transcription factor activity TAS - PMID:10051678
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
PBM	Zoo_01		Inferred - NFAT5 (99% AA Identity, Gallus gallus)

Structure

Structure	PDB	1IMH

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 363, "endStyle": "jagged", "aliStart": 206, "text": "RHD", "colour": "#2cb42c", "aliEnd": 343, "start": 206, "href": "http://pfam.xfam.org/family/PF00554.20", "type": "pfama", "display": "true", "metadata": {"end": 363, "description": "Proteins containing the Rel homology domain (RHD) are eukaryotic transcription factors. ", "database": "PfamA", "aliStart": 206, "scoreName": "E-value", "accession": "PF00554.20", "start": 206, "score": 4.3e-20, "identifier": "Rel homology DNA-binding domain", "type": "DBD", "aliEnd": 343}}, {"startStyle": "straight", "end": 469, "endStyle": "straight", "aliStart": 370, "text": "RHD_dimer", "colour": "#9999ff", "aliEnd": 468, "start": 370, "href": "http://pfam.xfam.org/family/PF16179.3", "type": "pfama", "display": "true", "metadata": {"end": 469, "description": "The Rel homology domain (RHD) is composed of two structural domains, an N-terminal DNA_binding domain (Pfam:PF00554) and a C-terminal dimerisation domain. This is the dimerisation domain [1].", "database": "PfamA", "aliStart": 370, "scoreName": "E-value", "accession": "PF16179.3", "start": 370, "score": 1.2e-26, "identifier": "Rel homology dimerisation domain", "type": "DBD", "aliEnd": 468}}], "length": 1456}

{"regions": [{"startStyle": "curved", "end": 439, "endStyle": "jagged", "aliStart": 282, "text": "RHD", "colour": "#2cb42c", "aliEnd": 419, "start": 282, "href": "http://pfam.xfam.org/family/PF00554.20", "type": "pfama", "display": "true", "metadata": {"end": 439, "description": "Proteins containing the Rel homology domain (RHD) are eukaryotic transcription factors. ", "database": "PfamA", "aliStart": 282, "scoreName": "E-value", "accession": "PF00554.20", "start": 282, "score": 4.6e-20, "identifier": "Rel homology DNA-binding domain", "type": "DBD", "aliEnd": 419}}, {"startStyle": "straight", "end": 545, "endStyle": "straight", "aliStart": 446, "text": "RHD_dimer", "colour": "#9999ff", "aliEnd": 544, "start": 446, "href": "http://pfam.xfam.org/family/PF16179.3", "type": "pfama", "display": "true", "metadata": {"end": 545, "description": "The Rel homology domain (RHD) is composed of two structural domains, an N-terminal DNA_binding domain (Pfam:PF00554) and a C-terminal dimerisation domain. This is the dimerisation domain [1].", "database": "PfamA", "aliStart": 446, "scoreName": "E-value", "accession": "PF16179.3", "start": 446, "score": 1.2e-26, "identifier": "Rel homology dimerisation domain", "type": "DBD", "aliEnd": 544}}], "length": 1532}

{"regions": [{"startStyle": "curved", "end": 457, "endStyle": "jagged", "aliStart": 300, "text": "RHD", "colour": "#2cb42c", "aliEnd": 437, "start": 300, "href": "http://pfam.xfam.org/family/PF00554.20", "type": "pfama", "display": "true", "metadata": {"end": 457, "description": "Proteins containing the Rel homology domain (RHD) are eukaryotic transcription factors. ", "database": "PfamA", "aliStart": 300, "scoreName": "E-value", "accession": "PF00554.20", "start": 300, "score": 4.6e-20, "identifier": "Rel homology DNA-binding domain", "type": "DBD", "aliEnd": 437}}, {"startStyle": "straight", "end": 563, "endStyle": "straight", "aliStart": 464, "text": "RHD_dimer", "colour": "#9999ff", "aliEnd": 562, "start": 464, "href": "http://pfam.xfam.org/family/PF16179.3", "type": "pfama", "display": "true", "metadata": {"end": 563, "description": "The Rel homology domain (RHD) is composed of two structural domains, an N-terminal DNA_binding domain (Pfam:PF00554) and a C-terminal dimerisation domain. This is the dimerisation domain [1].", "database": "PfamA", "aliStart": 464, "scoreName": "E-value", "accession": "PF16179.3", "start": 464, "score": 1.3e-26, "identifier": "Rel homology dimerisation domain", "type": "DBD", "aliEnd": 562}}], "length": 1549}

TF Info Page for NFAT5 (Rel)

Conclusion

Description

Previous Annotations

DNA-Binding

Published Motif Data

Structure

Experimental History

External Contribution