TF Info Page for NFAT5 (Rel)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: nuclear factor of activated T-cells 5, tonicity-responsive [Source:HGNC Symbol;Acc:HGNC:7774]
Entrez Summary The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000102908
External Link: CisBP
Interpro IPR002909; IPR008366; IPR008967; IPR011539; IPR014756; ;
Protein Domain: ENSP00000338806
Protein Domain: ENSP00000346420
Protein Domain: ENSP00000377343
Protein Domain: ENSP00000455628
Protein Domain: ENSP00000457593
Protein Domain: ENSP00000463214
Protein Domain: ENSP00000486610
Domain:
Protein: ENSP00000338806DBD: RHDOther: RHD_dimer
Protein: ENSP00000346420DBD: RHDOther: RHD_dimer
Protein: ENSP00000377343DBD: RHDOther: RHD_dimer
Protein: ENSP00000455628DBD: RHDOther: RHD_dimer
Protein: ENSP00000457593DBD: RHDOther: RHD_dimer
Protein: ENSP00000463214DBD: RHDOther:
Protein: ENSP00000486610DBD: RHDOther: RHD_dimer

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:10377394 11485737 16027109
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:11485737
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:10051678
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMZoo_01Inferred - NFAT5 (99% AA Identity, Gallus gallus)

Structure

Structure PDB 1IMH

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution